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GenomicsHow C/EBPa creates an elite cell state for reprogramming to pluripotency [ChIPseq]
Mouse Bcell, upon ectopic expression of the transcription factor Cebpa for 18h, can be reprogrammed to iPS with extremely high efficiency. To understand the molecular control of this phenomena we performed multiple high throughtput functionnal genomic analysis. Genome binding/occupancy of transcript...
ORGANISM(S): Mus musculus 
2016-02-19 | E-GEOD-71215 | biostudies-arrayexpress
TranscriptomicsEvaluation of a non-animal toolbox informed by adverse outcome pathways (AOPs) for human inhalation safety
Concentration- and time- response and human lower EpiAveolar™ respiratory tract tissues comparison of transcriptional responses following 12-day exposure with different test materials. Differential expression and pathway analyses were performed to estimate the lowest transcriptional point of depar...
ORGANISM(S): Homo sapiens 
2025-01-19 | E-MTAB-14272 | biostudies-arrayexpress
TranscriptomicsHow C/EBPa creates an elite cell state for reprogramming to pluripotency [4C]
Mouse Bcell, upon ectopic expression of the transcription factor Cebpa for 18h, can be reprogrammed to iPS with extremely high efficiency. To understand the molecular control of this phenomena we performed multiple high throughtput functionnal genomic analysis. Circularized chromosome conformation c...
ORGANISM(S): Mus musculus 
2016-02-19 | E-GEOD-75481 | biostudies-arrayexpress
TranscriptomicsHow C/EBPa creates an elite cell state for reprogramming to pluripotency [ATACseq]
Mouse Bcell, upon ectopic expression of the transcription factor Cebpa for 18h, can be reprogrammed to iPS with extremely high efficiency. To understand the molecular control of this phenomena we performed multiple high throughtput functionnal genomic analysis. Chromatin accessibility by ATACseq in ...
ORGANISM(S): Mus musculus 
2016-02-19 | E-GEOD-71214 | biostudies-arrayexpress
TranscriptomicsHow C/EBPa creates an elite cell state for reprogramming to pluripotency [RNAseq]
Mouse Bcell, upon ectopic expression of the transcription factor Cebpa for 18h, can be reprogrammed to iPS with extremely high efficiency. To understand the molecular control of this phenomena we performed multiple high throughtput functionnal genomic analysis. Transcriptomic by RNAseqencing (polyA+...
ORGANISM(S): Mus musculus 
2016-02-19 | E-GEOD-71217 | biostudies-arrayexpress
TranscriptomicsDNA sequencing to validate random deletions in Mycoplasma pneumoniae
We present LoxTnSeq, a new methodology to generate and catalogue libraries of genome reduction mutants. LoxTnSeq combines random integration of Lox sites by transposon mutagenesis, and the generation of mutants via cre recombinase, catalogued via deep-sequencing. When LoxTnSeq was applied to the nat...
ORGANISM(S): Mycoplasma pneumoniae 
2021-08-18 | E-MTAB-9582 | biostudies-arrayexpress
TranscriptomicsHow C/EBPa creates an elite cell state for reprogramming to pluripotency
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Mus musculus 
2016-02-19 | E-GEOD-71218 | biostudies-arrayexpress
TranscriptomicsHow C/EBPa creates an elite cell state for reprogramming to pluripotency [array]
Mouse Bcell, upon ectopic expression of the transcription factor Cebpa for 18h, can be reprogrammed to iPS with extremely high efficiency. To understand the molecular control of this phenomena we performed multiple high throughtput functional genomic analysis. Transcriptomic by microarray in Bcell, ...
ORGANISM(S): Mus musculus 
2016-02-19 | E-GEOD-71213 | biostudies-arrayexpress
GenomicsTransposon random mutagenesis of Mycoplasma pneumoniae M129 using ProTInSeq protocol along different concentrations and using two positive selection markers (Chloramphenicol acetyltransferase and Erythromycin esterase EreA) and a negative selection marker (barnase)
Definition of different Tn4001-derived mini-transposons carrying reporters with no translation initiation codons so they can only be expressed when fused to an endogenous protein. We used Chloramphenicol acetyltransferase (Cm) as a positive selection marker while the RNAse barnase (Barn) was used as...
ORGANISM(S): Mycoplasma pneumoniae M129 
2023-07-31 | E-MTAB-10380 | biostudies-arrayexpress
GenomicsBiosafety circuits for Mycoplasma - DNA-seq for passages 2, 3 and 15 of clonal Mycoplasma pneumoniae biosafety strains to study long-term stability of kill-switch devices
We have engineered synthetic gene switches to control and limit Mycoplasma growth for biosafety containment applications. Mycoplasmas have high mutation rates and, the accumulation of mutations that inactivate the circuit is expected. However, the question is how resilient is the kill-switch to muta...
ORGANISM(S): Mycoplasma pneumoniae M129 
2022-02-09 | E-MTAB-10981 | biostudies-arrayexpress
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