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To elucidate the mechamisms of colonic Treg cell induction by microbial metabolite(s), chroloform-resistant bacteria (CRB)-associated mice was developed and given low-fiber diet (LFD) and high-fiber diet (HFD). The colonic epithelial cells were isolated and gene expression profiles were analyzed by ...
ORGANISM(S): Mus musculus 
To investigate the molecular mechanism how butyrate promoter differentiation of naïve T cells into regulatory T cells Examination of acetylated histone H3 during the differentiation of regulatory T cells in the presence or absence of butyrate
ORGANISM(S): Mus musculus 
Commensal bacteria shapes gut immune system. Colonization bacteria increase the frequency of regulatory T cells, however, the molecular mechanisms has not yet been unknown. To reveal the mechanism, we isolated NaM-CM-/ve CD4+ T cells from spleen of C57BL/6 mice and cultured the cells under Treg-indu...
ORGANISM(S): Mus musculus 
Caecal contents from three specific pathogen-free (SPF) mice, and three germ-free (GF) mice were analyzed by DIA-MS (SWATH-MS) using TripleTOF 5600+ (SCIEX). Paraprevotella clara (P. clara) culture supernatants nontreated and treated tunicamycin or 2-fluro-L-fucose (2F-Fuc) were analyzed by DIA-MS u...
ORGANISM(S): Paraprevotella Clara Mus Musculus (mouse) 
00502 protein derived from Paraprevotella clara, as well as mixture of 00502 protein and human PRSS2 were analyzed by Native-PAGE. The observed bands were cut out and digested in-gel. The digested peptides were analyzed by DDA-MS using Q Exactive HF-X .
ORGANISM(S): Paraprevotella Clara 
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