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ProteomicsA safe alternative to H37Rv: the attenuated double auxotroph Mycobacterium tuberculosis ΔleuDΔpanCD
Currently available model organisms such as Mycobacterium smegmatis and Mycobacterium bovis Bacillus Calmette-Guérin (BCG) have significantly contributed to our understanding of tuberculosis (TB) biology, these models have limitations such as differences in genome size, growth rates and virulence. A...
ORGANISM(S): Mycobacterium tuberculosis 
2019-07-30 | PXD013677 | Pride
Biosafety level 2 Model organism Mycobacterium tuberculosis Attenuated auxotroph 
ProteomicsFeasibility of protein turnover studies in prototroph yeast (auxotroph data)
uantitative proteomics studies of yeast that use metabolic labeling with amino acids rely on auxotrophic mutations of one or more genes on the amino acid biosynthesis pathways. These mutations affect yeast metabolism, and preclude the study of some biological processes. Overcoming this limitation, i...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-02-02 | PXD005795 | Pride
Yeast Prototroph Turnover Protein stability Auxotroph Pulsed silac 
UnknownA uracil auxotroph Toxoplasma gondii exerting immunomodulation to inhibit breast cancer growth and metastasis.
Not available
S-EPMC8665513 | biostudies-literature
UnknownL-Alanine Prototrophic Suppressors Emerge from L-Alanine Auxotroph through Stress-Induced Mutagenesis in Escherichia coli.
Not available
S-EPMC7996224 | biostudies-literature
TranscriptomicsTranscriptional profiling by array of Arabidopsis ABA auxotroph to identify early downstream targets of transcription in response to a small dose of abscisic acid (ABA)
Abscisic acid (ABA) is a plant hormone that is important in responding to various environmental stresses. Using an ABA auxotroph in Arabidopsis (aba2-2) as the plant material, we would like to identify early downstream targets of transcription in response to a small dose of ABA, 1 uM. We also added ...
ORGANISM(S): Arabidopsis thaliana 
2011-10-30 | E-MEXP-3404 | biostudies-arrayexpress
UnknownStructural specificity of the spermidine requirement of an Escherichia coli auxotroph.
Not available
S-EPMC293647 | biostudies-literature
UnknownBrucella abortus strain RB51 leucine auxotroph as an environmentally safe vaccine for plasmid maintenance and antigen overexpression.
Not available
S-EPMC2583484 | biostudies-literature
GenomicsFOCUS
Formate-dependent evolved isolates of an Escherichia coli K12 energy-auxotroph
PRJEB97260 | ENA
TranscriptomicsTranscriptional profile of an eca39∆ mutant fission yeast
An branched-chain amino acids auxotroph eca39∆ mutant fission yeast exhibits an unusual adaptive growth phenotype on solid minimal media containing Ile, Leu and Val when other strains are growing nearby. The transcriptional profiles of an eca39∆ mutant before and after the adaptation were determined...
ORGANISM(S): Schizosaccharomyces pombe 
2012-09-21 | E-GEOD-29355 | biostudies-arrayexpress
UnknownThe semiquinone at the Qi site of the bc1 complex explored using HYSCORE spectroscopy and specific isotopic labeling of ubiquinone in Rhodobacter sphaeroides via (13)C methionine and construction of a methionine auxotroph.
Not available
S-EPMC4179594 | biostudies-literature
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