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GenomicsBLIMP1 ChIPseq
As a part of a modelling experiment for transcriptional control of mouse primordial germ cell specification, the transcription factor BLIMP1 was transiently expressed in the mouse p19 embryonal carcinoma cell line and its genome wide binding sites were defined using ChIPseq.
ORGANISM(S): Mus musculus 
2013-07-17 | E-MTAB-1599 | biostudies-arrayexpress
GenomicsAP2gamma ChIPseq
As a part of a modelling experiment for transcriptional control of mouse primordial germ cell specification, the transcription factor AP2gamma was stably expressed in the mouse p19 embryonal carcinoma cell line and its genome wide binding sites were defined using ChIPseq.
ORGANISM(S): Mus musculus 
2013-07-17 | E-MTAB-1600 | biostudies-arrayexpress
GenomicsNANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers [ChIP-seq]
Nanog, a core pluripotency factor in the inner cell mass of blastocysts, is also expressed in unipotent primordial germ cells (PGC) in mice1, where its precise role is yet unclear2-4. We investigated this in an in vitro model, where naïve pluripotent embryonic stem cells (ESCs) cultured in bFGF/Act...
ORGANISM(S): Mus musculus 
2016-01-11 | E-GEOD-71932 | biostudies-arrayexpress
UnknownInitiation of epigenetic reprogramming of the X chromosome in somatic nuclei transplanted to a mouse oocyte.
Not available
S-EPMC1369137 | biostudies-literature
TranscriptomicsPRMT5 protects genomic integrity during global DNA demethylation in primordial germ cells and preimplantation embryos
Primordial germ cells (PGCs) and preimplantation embryos undergo epigenetic reprogramming, which entails comprehensive erasure of DNA methylation. We found that PRMT5, an arginine methyltransferase, translocates from the cytoplasm to the nucleus during this process. Here we show that conditional los...
ORGANISM(S): Mus musculus 
2014-09-09 | E-GEOD-61216 | biostudies-arrayexpress
TranscriptomicsNANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers
Nanog, a core pluripotency factor in the inner cell mass of blastocysts, is also expressed in unipotent primordial germ cells (PGC) in mice1, where its precise role is yet unclear2-4. We investigated this in an in vitro model, where naïve pluripotent embryonic stem cells (ESCs) cultured in bFGF/Act...
ORGANISM(S): Mus musculus 
2016-01-11 | E-GEOD-71933 | biostudies-arrayexpress
TranscriptomicsPrimordial germ cell like cell competent populations
We sequenced human embryonic stem cells (hESCs), pre-mesendoderm cells (PreME) that acquire transient competence for PGCLC specification and cells at the mesendoderm (ME) stage when they are not longer PGC-competent.
ORGANISM(S): Homo sapiens 
2022-01-21 | E-MTAB-11135 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of Homo sapiens differentiated primordial germ cell-like cell derived from embryoid bodies
We sequenced embryoid bodies at various time points following induction of pre-mesendoderm cells (PreME) towards primordial germ cell-like cell (PGCLC) fate
ORGANISM(S): Homo sapiens 
2023-05-05 | E-MTAB-11283 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of embryonal carcinoma cell line P19 with or without expressoin of Blimp1-EGFP fusion protein over a 48-hour time-course to study the role of Blimp1 in transcription regulation in pluripotent embryonic cells
Genome-wide expression analysis was performed with the aim of investigating transcriptional changes mediated by the transcription factor B lymphocyte-induced maturation protein-1 (Blimp1) in the context of pluripotent embryonic cells, with the aim of drawing parallels to its functions in mouse primo...
ORGANISM(S): Mus musculus 
2013-05-11 | E-MTAB-865 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by high throughput sequencing of primordial germ cells from e6.5, e7.5, e8.5 embryos and e7.5 somatic neighbours and Blimp1-KO mice
Primordial germ cell mRNA profiles from cells microdissected from e6.5, e7.5 and e8.5 embryos, e7.5 somatic neighbours and Blimp1-KO mice were generated by single cell library construction and sequencing in duplicate using Applied Biosystems SOLiD sequencer. Single cell library construction is descr...
ORGANISM(S): Mus musculus 
2013-07-01 | E-MTAB-1178 | biostudies-arrayexpress
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