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GenomicsChIP-Seq of human embryonic stem cells of a differentiation time course with antibody against H3K4me3
H3K4me3 ChIP-Seq profiling of a directed differentiation time course converting human embryonic stem cells (hES) into immature pancreatic beta cell precursors. CyT49, a Viacyte proprietary male hESC line with normal karyotype was used.
ORGANISM(S): Homo sapiens 
2012-10-13 | E-MTAB-821 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of a directed differentiation time course converting human embryonic stem cells (hES) into immature pancreatic beta cell precursors
Transcriptional profiling of a directed differentiation time course converting human embryonic stem cells (hES) into immature pancreatic beta cell precursors.
ORGANISM(S): Homo sapiens 
2012-10-11 | E-MTAB-817 | biostudies-arrayexpress
TranscriptomicsmicroRNA profiling by array of a directed differentiation time course converting human embryonic stem cells (hES) into immature pancreatic beta cell precursors
miRNA transcript profiling of a directed differentiation time course converting human embryonic stem cells (hES) into immature pancreatic beta cell precursors.
ORGANISM(S): Homo sapiens 
2012-10-11 | E-MTAB-818 | biostudies-arrayexpress
GenomicsChIP-seq of wild-type SOX18 and a dominant-negative variant (SOX18RaOp) in HeLa cells
We investigated differences in the chromatin-binding profile of SOX18 (mouse) and its dominant-negative isoform SOX18RaOp, and how SOX18RaOp alters the binding location of SOX18. HeLa cells were transfected to generate 3 different conditions: 1. myc-SOX18, 2. myc-SOX18RaOp and 3. myc-SOX18 with unta...
ORGANISM(S): Homo sapiens 
2021-08-30 | E-MTAB-10609 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human imatinib-sensitive and imatinib-resistant cells compared in the absence of imatinib
Both imatinib-sensitive and imatinib-resistant cells were compared in the absence of imatinib. The resistant cells in the presence of imatinib were compared to the sample in the absence of imatinib. The sample from the resistant cells in the presence of imatinib was labelled in duplicate to assess f...
ORGANISM(S): Homo sapiens 
2003-11-30 | E-MEXP-21 | biostudies-arrayexpress
TranscriptomicsSingle cell RNA sequencing of stem cell-derived retinal ganglion cells
We used human embryonic stem cell-derived retinal ganglion cells (RGCs) to characterize the transcriptome of 1,174 cells at the single cell level. The human embryonic stem cell line BRN3B-mCherry A81-H7 was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 3...
ORGANISM(S): Homo sapiens 
2017-10-07 | E-MTAB-6108 | biostudies-arrayexpress
TranscriptomicsRNA seq of rectal tumour tissue and paired healthy tissue, with EpCAM purification
Tumour tissue was immediately obtained post-surgery along with paired healthy tissue near (<2cm from tumour margin) and far (>10cm) from the tumour site. Each tissue was dissociated and stained with flow cytometry antibodies and sorted by FACS into Live/Dead-negative EpCAM-positive CD3-negative popu...
ORGANISM(S): Homo sapiens 
2020-02-27 | E-MTAB-8803 | biostudies-arrayexpress
TranscriptomicsRiboSeq of African green monkey (Vero) cells infected at a multiplicity of infection (MOI) of 3 with the Asian/American Zika virus (ZIKV) strain, PE243
Vero cells were infected with the Asian/American Zika virus (ZIKV) strain, PE243, at MOI:3 to assess the viral transcriptome. Samples were harvested at 24 hours post-infection (h p.i.) by flash-freezing, without cycloheximide pre-treatment. RNase I treatment was carried out, following which ribosome...
ORGANISM(S): Chlorocebus sabaeus 
2024-09-04 | E-MTAB-12971 | biostudies-arrayexpress
TranscriptomicsComparative genomic hybridization of benign epithelial and prostate cancer cell lines derived from the same patient
Copy number alterations in a matched pair of benign epithelial and prostate cancer cell lines derived from the same patient were assessed using array-based comparative genomic hybridisation (aCGH). the cancer cell line showed a gain of chromosome 7, deletion of chromosome 8, gains and losses in chro...
ORGANISM(S): Homo sapiens 
2006-08-04 | E-MEXP-803 | biostudies-arrayexpress
TranscriptomicsRiboSeq of African green monkey (Vero) cells infected at a multiplicity of infection (MOI) of 3 with different Zika virus (ZIKV) mutants (American WT, African-like, uORF1-KO and uORF2-PTC1) generated by reverse genetics
Vero cells were infected with the different Zika virus (ZIKV) mutants (American WT, African-like, uORF1-KO and uORF2-PTC1) generated by reverse genetics at MOI:3 to assess their viral transcriptome. Samples were harvested at 24 hours post-infection (h p.i.) by flash-freezing, without cycloheximide p...
ORGANISM(S): Chlorocebus sabaeus 
2024-09-04 | E-MTAB-12969 | biostudies-arrayexpress
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