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TranscriptomicsIsolation and in vitro expansion of human colonic stem cells
This SuperSeries is composed of the following subset Series: GSE31255: Isolation and in vitro expansion of human colonic stem cells [Expression profile] GSE31256: Isolation and in vitro expansion of human colonic stem cells [CGH] Refer to individual Series
ORGANISM(S): Homo sapiens 
2011-11-22 | E-GEOD-31257 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNA sequencing of colorectal cancer
We profile single cells from patients with colorectum cancer using Chromium 3’ and 5’ single-cell RNA-sequencing. Patients EXT001, EXT009, and EXT012 from the KUL dataset were first analyzed by Lee et al., 2020, and the raw data are available in ArrayExpress under the accession codes E-MTAB-8410 and...
ORGANISM(S): Homo sapiens 
2022-09-16 | E-MTAB-9934 | biostudies-arrayexpress
TranscriptomicsIsolation and in vitro expansion of human colonic stem cells [CGH]
Tissue for normal colonic stem cell isolation was obtained via colectomy from a colorectal cancer patient. It was shown that the resected tissue and the in vitro-cultured organoids grown from tissue-derived adult stem cells do not harbor chromosomal alterations. For that purpose, genomic DNA from re...
ORGANISM(S): Homo sapiens 
2011-11-22 | E-GEOD-31256 | biostudies-arrayexpress
TranscriptomicsIsolation and in vitro expansion of human colonic stem cells [Expression profile]
Using the surface marker EPHB2, we have FACS-purified and profiled stem cell-enriched cell fractions from normal human mucosa, crypt proliferative progenitors and late transient amplifying cells to define a gene expression program specific for normal human colon epithelial stem cells We FACS purifie...
ORGANISM(S): Homo sapiens 
2011-11-22 | E-GEOD-31255 | biostudies-arrayexpress
TranscriptomicsCRC micrometastasis generated by AKP MTOs resolved by single cell RNAseq
Longitudinal single cell RNA-expression analysis of tumor cells along metastatic relapse. AKP mouse tumor organoids (MTOs) were implanted in the caecum and left to metastasize into the liver. Livers were collected at different timepoints post-injection and GFP+ CD45- cells were sorted in 96-well sma...
ORGANISM(S): Mus musculus 
2022-11-18 | E-MTAB-11981 | biostudies-arrayexpress
TranscriptomicsThe intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse
Using EphB2 or the ISC marker Lgr5, we have FACS-purified and profiled intestinal stem cells (ISCs), crypt proliferative progenitors and late transient amplifying cells to define a gene expression program specific for normal ISCs. A frequent complication in colorectal cancer (CRC) is regeneration of...
ORGANISM(S): Mus musculus 
2011-03-14 | E-GEOD-27605 | biostudies-arrayexpress
TranscriptomicsSpatiotemporal dynamics of CRC metastases resolved by single cell RNAseq
Longitudinal single cell RNA-expression analysis of tumor cells along the metastatic cascade. AKTP MTOs were implanted in the caecum and left to metastasize into the liver. Mice with different metastatic burden were collected and GFP+ CD45- cells were sorted in 96-well smart-seq plates. Tumor cells ...
ORGANISM(S): Mus musculus 
2022-09-16 | E-MTAB-11302 | biostudies-arrayexpress
TranscriptomicsSingle cell profiling of CRC primary tumors generated by the injection of AKTP mouse tumor organoid
The objective of this scRNAseq was to explore the heterogeneity between tumor epithelial CRC cells in our AKTP mouse models. We compared it to human datasets and found that mouse and human CRCs share similar tumor subpopulations.
ORGANISM(S): Mus musculus 
2022-09-16 | E-MTAB-11284 | biostudies-arrayexpress
TranscriptomicsIdentification of beta-cetenin/TCF4 target genes in LS174T colorectal cancer cells using gene expression arrays
Aberrant activation of WNT signaling and loss of BMP signals represent the two main alterations leading to the initiation of colorectal cancer (CRC). Here we screen for genes required for maintaining the tumor stem cell phenotype and identify the zinc-finger transcription factor GATA6 as key regulat...
ORGANISM(S): Homo sapiens 
2014-05-20 | E-GEOD-56896 | biostudies-arrayexpress
TranscriptomicsExpression profiles of cell populations purified from human CRC (3 ways)
The survival of isolated metastatic cells and expansion into macroscopic tumour has been recognized as a limiting step for metastasis formation in several cancer types yet the determinants of this process remain largely uncharacterized. In colorectal cancer (CRC), we identify a transcriptional progr...
ORGANISM(S): Homo sapiens 
2013-01-17 | E-GEOD-39395 | biostudies-arrayexpress
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