E. coli MG1655 containing the plasmids pBRA SP-Tox-Act1WT or SP-Tox-Act1C151A were cultured in LB and resuspended in AB supplemented with 0.2% L-arabinose to induce Tox-Act1 expression. Cell pellets were resuspended and the lipid-containing phase was extracted. Lipid extracts were analyzed using ult...
ORGANISM(S): Escherichia Coli K-12
