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Optimisation of a bead-beating procedure for simultaneous extraction of bacterial and fungal DNA from pig faeces and liquid feed for 16S and ITS2 rDNA amplicon sequencing
We investigated the effect of homogenization strategy and protein precipitation on downstream protein quantitation using multiple reaction monitoring mass spectrometry (MRM-MS). Our objective was to develop a workflow capable of processing disparate tissue types with high throughput, minimal variabi...
ORGANISM(S): Mus Musculus 
2021-01-24 | PXD019539 | panorama
Genomics
Impact of bead-beating intensity on gut microbiome detection by 16S sequencing
Genomics
Impact of bead-beating time on microbiome recovery in mouse and human stool-16S sequencing data
Human stool samples were collected, microbial cells were harvested and subjected to protein extraction using different protocols for metaproteomics analyses. The protein extraction methods differ from the usage of different lysis buffer (SDS, urea and commercially available B-Per reagent), bead beat...
ORGANISM(S): human gut metagenome 
2017-07-17 | PXD005969 | Pride
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