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Transcriptomics3' RNA-Seq (3'SEQ) analysis gene expression profiling at G1-S transition in human embryonic stem cells (hESCs) and hESC-derived endoderm cells
In this report, we examine transcripts on a genome-wide level between the synchronized cell cycles of hESCs and hESC-derived endoderm cells. We found 10347, 10299 and 10362 genes expressed in the G1, G1/S, and S phase of hESCs, respectively; 10333, 10227 and 10215 genes expressed at the G1, G1/S and...
ORGANISM(S): Homo sapiens 
2014-05-14 | E-GEOD-47117 | biostudies-arrayexpress
TranscriptomicsTranscriptome of HEK293 cells stably knockdown for the BAHD1 gene with a shRNA
Comparison of gene expression profile of HEK293 cells stably expressing a shRNA control (SilX-CT) or a shRNA against BAHD1 (SilX-BAHD1) We used microarrays to identify the repertoire of genes differentially expressed upon knockdown of the chromatin repressor BAHD1 in human HEK293 cells.
ORGANISM(S): Homo sapiens 
2015-12-11 | E-GEOD-53284 | biostudies-arrayexpress
TranscriptomicsmicroRNA expression profiles of distinct human mammary epithelial cell types
RNA samples were prepared from freshly sorted mammary cell subpopulations (MaSC/basal-enriched, luminal progenitor, mature luminal and stromal) from five human donors. High throughput RT-PCR was used to measure the global microRNA expression profiles of each cell subpopulation. The expression profil...
ORGANISM(S): Homo sapiens 
2016-02-20 | E-GEOD-67054 | biostudies-arrayexpress
TranscriptomicsmicroRNA expression profiles of distinct mouse mammary epithelial cell types
RNA samples were prepared from freshly sorted mammary cell subpopulations (MaSC/basal-enriched, luminal progenitor, mature luminal and stromal) from five sets of adult mice. High throughput RT-PCR was used to measure the global microRNA expression profiles of each cell subpopulation. The expression ...
ORGANISM(S): Mus musculus 
2016-02-20 | E-GEOD-67055 | biostudies-arrayexpress
TranscriptomicsmicroRNA expression profiles of distinct human and mouse mammary epithelial cell types
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Mus musculus 
2016-02-20 | E-GEOD-67056 | biostudies-arrayexpress
TranscriptomicsTranscriptional profiling of Escherichia coli after addition of CO-RMs to anaerobically growing cells
Escherichia coli strain MG1655 was grown to mid-log phase in defined anaerobic media. One sample was treated with 100 µM CORM-3, the other sample was a control. The volume (175 ml), temperature (37oC) and stirring (200 rpm) were constant. After 15 min of exposure to CO-RM, samples were taken from t...
ORGANISM(S): Escherichia coli 
2010-06-24 | E-GEOD-12878 | biostudies-arrayexpress
TranscriptomicsHOP homeobox (Hopx) and Histone deacetylase-2 (Hdac2) deficiency effect on the embryonic heart
Analysis of heart ventricles from Hopx, Hdac2, and both Hopx-Hdac2 deficient embryos at embryonic day E16.5. Results provide insight into the role of Hopx and Hdac2 in cardiac development. We used microarrays to detail the global programme of gene expression underlying cardiac development by Hopx an...
ORGANISM(S): Mus musculus 
2010-08-19 | E-GEOD-23700 | biostudies-arrayexpress
TranscriptomicsmCasz1_conditional knockout
The overall goal of our studies is to elucidate the cellular and molecular mechanism by which the transcription factor Casz1 functions in murine heart development. We established that Casz1 is expressed in myocardial progenitor cells beginning at E7.5 and in differentiated cardiomyocytes throughout ...
ORGANISM(S): Mus musculus 
2015-05-15 | E-GEOD-55394 | biostudies-arrayexpress
TranscriptomicsRNA-sequencing of unstressed and pressure-overloaded C57 mouse hearts with single or combined ablation of PKCdelta and PKCepsilon
Combinatorial ablation of the major cardiac-expressed novel protein kinase C isoforms, PKCdelta and PKCepsilon, in murine cadiac myocytes unmasked functionally redundant growth-limiting effects of these kinases in hemodynamically stressed adult hearts and during normal embryonic cardiac development,...
ORGANISM(S): Mus musculus 
2015-04-21 | E-GEOD-62689 | biostudies-arrayexpress
TranscriptomicsEgress of mature murine regulatory T cells from the thymus requires RelA
The mechanism of egress of mature regulatory T cells (Tregs) from the thymus to the periphery remains enigmatic, as does the nature of those factors expressed in the thymic environment. Here, we examined the fate of thymic Tregs in TNFα/RelA double-knockout (TA-KO) mice, because TA-KO mice retain a...
ORGANISM(S): Mus musculus 
2015-03-04 | E-GEOD-64191 | biostudies-arrayexpress
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