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TranscriptomicsRNA-seq of Chlamydomonas reinhardtii under diel conditions to identify cycling genes
Biological rhythms in response to both endogenous (circadian) and exogenous (e.g. diel) cycles, with a period of ~24 hours, are a prominent feature of many living systems. In green algal species, knowledge on the extent of diel rhythmicity of genome wide gene expression, its evolution, and its cis-r...
ORGANISM(S): Chlamydomonas reinhardtii 
2014-10-25 | E-GEOD-62671 | biostudies-arrayexpress
TranscriptomicsGlobal Changes following N-deprivation in Chlamydomonas: 454 sequencing
Chlamydomonas reinhardtii forms lipid droplets rich in triacylglycerols when nutrient-deprived. To begin studying the mechanisms underlying this process, N-deprivation was used to induce triacylglycerol accumulation and changes in developmental programs such as gametogenesis. Comparative global anal...
ORGANISM(S): Chlamydomonas reinhardtii 
2010-09-24 | E-GEOD-24366 | biostudies-arrayexpress
TranscriptomicsRNA-seq of Chlamydomonas reinhardtii response to nitrogen deprivation
Chlamydomonas reinhardtii forms lipid droplets rich in triacylglycerols when nutrient-deprived. To begin studying the mechanisms underlying this process, N-deprivation was used to induce triacylglycerol accumulation and changes in developmental programs such as gametogenesis. Comparative global anal...
ORGANISM(S): Chlamydomonas reinhardtii 
2010-09-24 | E-GEOD-24365 | biostudies-arrayexpress
TranscriptomicsChanges in membrane lipid composition during Iron depletion and starvation in Chlamydomonas reinhardtii
C. reinhardtii cells exposed to abiotic stresses (e.g. iron-, nitrogen-, zinc- or phosphorus-deficiency) accumulate TAGs which are stored in lipid droplets. Here, we report that iron starvation leads to formation of lipids bodies and accumulation of TAGs. This occurs between 12 and 24 h of iron-star...
ORGANISM(S): Chlamydomonas reinhardtii 
2014-02-20 | E-GEOD-44611 | biostudies-arrayexpress
TranscriptomicsCarbon Redistribution from Surface Lipids into Triacylglycerols in Leaves of Arabidopsis by Introducing a Chlamydomonas Diacylglycerol Acyltransferase 2
Plants and microalgae have the ability to harness sunlight into energy dense molecules such as triacylglycerols (TAGs). TAGs hold great importance for human and animal nutrition, and are also a source for renewable energy. Acyl-CoA-dependent TAG synthesis by diacylglycerol acyltransferases (DGATs) i...
ORGANISM(S): Arabidopsis thaliana 
2014-06-16 | E-GEOD-38898 | biostudies-arrayexpress
TranscriptomicsSystems analysis of Chlamydomonas Cu nutrition reveals connections between Cu and multiple O2-dependent metabolic steps
We used digital gene expression (NlaIII sequence tags) and RNA-Seq to compare the transcriptomes of Cu-replete vs. Cu–deficient Chlamydomonas wild-type cells to reveal dozens of mRNAs whose abundance is modified. Half of the corresponding genes are targets of CRR1, a master regulator of nutritional ...
ORGANISM(S): Chlamydomonas reinhardtii 
2011-10-23 | E-GEOD-25124 | biostudies-arrayexpress
TranscriptomicsThe acclimation of Chlamydomonas reinhardtii to anaerobiosis involves plant and animal strategies
The absence of oxygen (O2) is a stress condition for aerobic organisms and requires extensive acclimation responses. Previously, Chlamydomonas reinhardtii has been used as a reference organism for understanding these acclimation responses. In this work, we use RNA-Seq for a whole genome view of the ...
ORGANISM(S): Chlamydomonas reinhardtii 
2013-07-01 | E-GEOD-42035 | biostudies-arrayexpress
TranscriptomicsTranscriptomic measurements of Nannochloropsis oceanica CCMP1779 through a light:dark cycle
Nannochloropsis oceanica CCMP1779 is a marine unicellular stramenopile and an emerging reference species for basic research on oleogenic microalgae with biotechnological relevance. We investigated its physiology and transcriptome under light/dark cycles. We observed oscillations in lipid content and...
ORGANISM(S): Nannochloropsis oceanica 
2015-08-01 | E-GEOD-69460 | biostudies-arrayexpress
TranscriptomicsSystems-level analysis of N-starvation induced modifications of carbon metabolism in a Chlamydomonas starchless mutant
To understand the molecular basis underlying increased triacylglycerol accumulation in starchless Chlamydomonas mutants, we undertook comparative time course transcriptomics of sta6 (CC-4348), a cw strain purported to represent the parental STA6 strain (CC-4349), and 3 independent STA6 strains (STA6...
ORGANISM(S): Chlamydomonas reinhardtii 
2013-10-24 | E-GEOD-51602 | biostudies-arrayexpress
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