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GenomicsHNF1A, H3K4me3 and H3K27ac ChIP-seq in control and Haster-deficient mouse livers
The HASTER promoter region is a cis-regulatory element that stabilizes the transcription of HNF1A, preventing silencing or overexpression. We have generated a mouse model where the promoter of Haster has been specifically deleted in liver (Haster loxP/loxP; AlbCre). In liver the prevailing conseque...
ORGANISM(S): Mus musculus 
2022-05-01 | E-MTAB-11471 | biostudies-arrayexpress
TranscriptomicsUMI-4C in control and HASTER-deficient EndoC-betaH3 cells overexpressing HNF1A
The HASTER promoter region is a cis-regulatory element that stabilizes the transcription HNF1A. If HASTER is deleted after beta cells have been formed and HNF1A is already stably expressed, HASTER is not required to maintain HNF1A activity, and is only required to provide negative feedback on HNF1A....
ORGANISM(S): Homo sapiens 
2022-05-01 | E-MTAB-11473 | biostudies-arrayexpress
TranscriptomicsStrain-specific RNA-seq in Haster Stop/+ C57BL/6;PWK/PhJ F1 mice
Germ-line deletion of the Haster promoter region results in overexpression of Hnf1a in liver, while a variegated phenotype is observed in pancreatic islets, with overexpression or silencing of the Hnf1a gene in different cells. Mouse hybrid experiments show that both activation and silencing mechani...
ORGANISM(S): Mus musculus 
2022-05-01 | E-MTAB-11475 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of pancreatic islets from control and Haster-pKO mice
Pancreas specific deletion of the Haster promoter region results in a variegated phenotype in pancreatic islets with overexpression or silencing of the Hnf1a gene. To determine the transcriptional consequence of the overexpression or silencing of Hnf1a is islet cells from the Haster pKO mice (Haster...
ORGANISM(S): Mus musculus 
2022-05-01 | E-MTAB-11477 | biostudies-arrayexpress
TranscriptomicsRNA-seq of MIN6 cells overexpressing Hnf1a through CRISPR activation
To determine the impact of Hnf1a overexpression on gene expression in mouse beta cells, we performed RNA-seq experiments on MIN6 where Hnf1a was overexpressed using CRISPR activation (SAM system).
ORGANISM(S): Mus musculus 
2022-05-01 | E-MTAB-11474 | biostudies-arrayexpress
TranscriptomicsUMI-4C in control and Haster-deficient mouse livers
The HASTER promoter region is a cis-regulatory element that stabilizes the transcription of HNF1A, preventing silencing or overexpression. We have generated a mouse model where the promoter of Haster has been specifically deleted in liver (Haster loxP/loxP; AlbCre). In liver the prevailing conseque...
ORGANISM(S): Mus musculus 
2022-05-01 | E-MTAB-11472 | biostudies-arrayexpress
TranscriptomicsRNA-seq in control and Haster-deficient mouse livers
The HASTER promoter region is a cis-regulatory element that stabilizes the transcription of HNF1A, preventing silencing or overexpression. We have generated a mouse model where the promoter of Haster has been specifically deleted in liver (Haster loxP/loxP; AlbCre). In liver the prevailing consequen...
ORGANISM(S): Mus musculus 
2022-05-01 | E-MTAB-11463 | biostudies-arrayexpress
GenomicsHuman Pancreatic Beta Cell lncRNAs Control Cell-Specific Regulatory Networks
Recent studies have uncovered thousands of long non-coding RNAs (lncRNAs) in human pancreatic β cells. β cell lncRNAs are often cell type specific and exhibit dynamic regulation during differentiation or upon changing glucose concentrations. Although these features hint at a role of lncRNAs in β cel...
EGAS00001002865 | EGA
GenomicsImperial College London - Beta Cell Genome Regulation Lab
Data Access Committee EGAC00001000853
EGAC00001000853 | EGA
Genomicsena-DATASET-ICL-01-03-2018-15:32:27:049-427 - samples
Whole Human Islet paired-ended RNA-seq of 64 human pancreatic donors.
EGAD00001003992 | EGA
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