FLAG-SRFR1 was expressed in reconstituted E. coli system (Okada el al 2009) in the presence of SUMO1 T91R SUMOylation-competent (containing diglycine, GG) or -deficient (diglycine replaced by dialanine, AA) isoforms, SCE1a, and SAE1/2. After induction of expressions, total extracts were probed with ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress)