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ProteomicsMudPIT analyses of the pyruvate dehydrogenase complex (PDC) isolated from Saccharomyces cerevisiae by FLAG-affinity purification and Size Exclusion Chromatography
Yeast protein extractions and subcellular fractionation Yeast cells expressing FLAG tagged proteins were grown in YPD medium at 30oC to OD600 of 0.8. 12 Liters YPD culture of cells were pelleted and washed in SB buffer. The pellet was resuspended in SB buffer and treated with 2 mg/ml Zymolyase 20T ...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2020-03-20 | MSV000085126 | MassIVE
ProteomicsMudPIT analyses of the pyruvate dehydrogenase complex (PDC) isolated from human
Mammalian protein extraction and Halo purification For Halo purification, HEK293T cells at 40% confluency in a 100 mm plate were transfected with 10ug of plasmid containing ORF of PDHA1-HA-Halo in 1X BBS with 125 mM CaCl2. Cells were harvested by scraping in PBS following PBS wash after 24 hours fro...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-03-20 | MSV000085125 | MassIVE
ProteomicsMudPIT analyses of the proteins associated with Human SETD2 deletion mutants
N-terminally Halo-tagged deletions of human SETD2 were constructed such as SETD2 ABCN1, A, B, C, CN, and CC consisted of amino acids 1-1692, 1-503, 504-1403, 1404-2564, 1404-1963, and 1964-2564, respectively. Deletion mutants were expressed in HEK293T cells and affinity purified, each in biological ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-06-08 | MSV000087587 | MassIVE
ProteomicsThe nuclear granule Paraspeckles interacts with the chromatin remodeling SWI/SNF complex to regulate alternative splicing
Protein samples were analyzed by Multidimensional Protein Identification Technology (MudPIT), as described previously. Briefly, precipitated proteins were resuspended in 30uL of 100mM Tris pH 8.5 with 8M urea to denature proteins. Cysteines were reduced and alkylated prior to digestion with recombi...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-04-04 | MSV000089198 | MassIVE
ProteomicsStructural basis of the interaction between SETD2 methyltransferase and hnRNP L paralogs for governing co-transcriptional splicing
Protein samples were analyzed by Multidimensional Protein Identification Technology (MudPIT), as described previously. Briefly, precipitated proteins were resuspended in 30uL of 100mM Tris pH 8.5 with 8M urea to denature proteins. Cysteines were reduced and alkylated prior to digestion with recombi...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-05-11 | MSV000087403 | MassIVE
ProteomicsWDR76-SPIN1_SCAP-E2-XL_mzTab and mgf
SCAP-XL of Halo-WDR76 and SNAP-SPIN1 from stable cell line in WDR76 KO background. Serial Capture Affinity Purification (SCAP) was performed on whole cell extract of a stable cell line of 293FRT cells in WDR76 KO background. DSSO crosslinked in E2 sample. Data was acquired using a ms1/ms2/ms3 method...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-01-22 | MSV000086749 | MassIVE
ProteomicsHalo-WDR76_Purification_WDR76KObackground_MudPIT_wPTMs
Halo purification samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits Halo-WDR76. Purification was performed using whole cell extract from a stable cell line of 293FRT cells in WDR76 KO background. Control purification was performed on bl...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-11 | MSV000086849 | MassIVE
ProteomicsHalo-Control/WDR76/SPIN1/CBX5_purification_MudPIT_wPTMs
Halo purification samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits are Halo-WDR76, Halo-SPIN1, Halo-CBX5. The bait for Control Purification is Halo tag only. Purification for each bait was performed using whole cell extract from a stab...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-11 | MSV000086846 | MassIVE
ProteomicsWDR76_SPIN1_Halo-AP_Enriched_Histones_AscendDIA
Histone PTM bottom-up profiling of Halo-WDR76 or Halo-SPIN1 Enriched or total histones (w Propionylation; no spike-in peptide) Supplementry data for WDR76-SPIN1 SCAP Analysis
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-04-15 | MSV000094548 | MassIVE
ProteomicsWDR76-SPIN1_inWDR76KO_SCAP-MS_MudPIT
Serial Capture Affinity Purification (SCAP) samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits are SNAP-SPIN1 and Halo-WDR76. SNAP purification was performed first, then Halo purification was performed. SCAP was performed using whole cel...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-03 | MSV000086802 | MassIVE
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