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MetabolomicsFunctional nutrient-genetic profiling reveals biotin and FBXW7 are essential to bypass glutamine addiction

Metabolic flexibility is key to survival and growth in all living organisms. In mammals, the pathways supporting cell proliferation in nutrient-limiting conditions have not been fully elucidated, although certain tumors display metabolic dependencies that can be targeted for therapy. He...

2026-02-02 | MTBLS13799 | MetaboLights
ProteomicsThiol-cleavable Biotin for Chemical and Enzymatic Biotinylation and its Application to Mitochondrial TurboID Proteomics
Protein biotinylation via chemical or enzymatic reactions is often coupled with streptavidin-based enrichment and on-beads digestion in numerous biological applications. However, the popular on-beads digestion method faces major challenges of streptavidin contamination, the lost information of bioti...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-04-21 | MSV000087256 | MassIVE
TranscriptomicsCatabolite repression of phosphoenolpyruvate carboxykinase by a zinc finger protein under biotin- and pyruvate carboxylase-deficient conditions in Pichia pastoris
We have identified a methanol- and biotin-starvation-inducible zinc finger protein named ROP [repressor of phosphoenolpyruvate carboxykinase (PEPCK)] in the methylotrophic yeast Pichia pastoris. When P.pastoris strain GS115 (wild-type, WT) is cultured in biotin-deficient, glucose ammonium (Bio-) ...
ORGANISM(S): Pichia pastoris 
2011-10-13 | E-GEOD-32948 | biostudies-arrayexpress
ProteomicsMudPIT analyses of the human proteins biotinylated by proximity to exogenously expressed biotin ligase (BioID)
To identify non-specific biotinylation events, HEK293 cells transfected with a plasmid carrying only the biotin ligase moiety were used as negative controls to BioID experiments. Cells were lysed in the presence of detergents. After purification of biotinylated proteins on streptavidin-conjugated be...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-30 | MSV000080809 | MassIVE
ProteomicsMicroID: A Novel Biotin Ligase Enables Rapid Proximity Ligation Proteomics
Identifying protein-protein interactions is central to dissecting signaling and regulatory processes in cells. BioID is a proximity ligation method that uses a promiscuous biotin ligase to detect protein-protein interactions in cells in a highly reproducible manner. Recent advancements in proximity ...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD031146 | Pride
Proximity ligation  bioid  turboid  organelle  mass spectrometry  microid 
ProteomicsMudPIT analyses of the human proteins biotinylated by proximity to dynein/dynactin subunits fused to biotin ligase (BioID)
We identified the human dynein proteome by attaching a promiscuous biotin ligase (BioID) to 7 distinct components of the dynein machinery, including a subunit of its essential cofactor dynactin. BioID experiments were performed with stable HEK293 cell lines expressing dynein (IC1, IC2, LIC1, LIC2, R...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080759 | MassIVE
ProteomicsMudPIT analyses of the human proteins biotinylated by proximity to biotin ligase (BioID) fused to the N-terminus of activators of the dynein/dynactin motor
To capture the spatial organization of the dynein/dynactin motor, we attached a promiscuous biotin ligase (BioID) to 6 distinct activators of the dynein machinery motile activity. BioID experiments were performed with stable HEK293 cell lines expressing full length BICD1, BICD2, HOOK1, HOOK3, NIN an...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-30 | MSV000080807 | MassIVE
GenomicsIdentification of G-quadruplex-interacting Proteins in Living Cells using an artificial G4-targeting Biotin ligase
Identification of G-quadruplex-interacting Proteins in Living Cells using an artificial G4-targeting Biotin ligase
PRJNA926896 | ENA
ProteomicsMudPIT analyses of the human proteins biotinylated by proximity to biotin ligase (BioID) fused to the C-terminus of activators of the dynein/dynactin motor
To understand how a single motor achieves cargo specificity, we attached a promiscuous biotin ligase (BioID) to 6 distinct activators of the dynein machinery motile activity, including two new activators, ninein and ninein-like. BioID experiments were performed with stable HEK293 cell lines expressi...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-30 | MSV000080808 | MassIVE
ProteomicsIdentification of MARCH2 E3 ligase-substrates using proximity-dependent biotin labeling method
MARCH2 is known to be involved in intracellular vesicular trafficking. To identify its substrates, we have recently developed a method based on proximity-dependent biotin labelling. In this protocol, the MARCH2 ubiquitin ligase of interest is expressed as a fusion to Escherichia coli biotin ligase ...
ORGANISM(S): Homo sapiens (Human) 
2019-06-06 | PXD013601 | Pride
March2 Secretory proteins E3 ligase 
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