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TranscriptomicsGene expression analysis of bovine oocytes with high developmental competence obtained from FSH-stimulated animals
The objective of the study was to analyze the impact of various lenght FSH withdrawal period (coasting) after the last FSH injection on transcriptome changes in in vivo bovine oocytes at the GV stage. Oocytes were collected from super-stimulated animals after 20, 44, 68 and 92 hours of coasting. For...
ORGANISM(S): Bos taurus 
2013-06-21 | E-GEOD-38345 | biostudies-arrayexpress
TranscriptomicsGranulosa cell differentiation in vivo and developmental competence of oocytes in the bovine model
Transcriptional profiling of in vivo granulosa cells collected at 20h, 44h, 68h, and 92h of coasting in lactating cow (breed Holstein) 4 conditions experiment, 3 biological replicates, one repliacte per array, dye swap experiment, two colors, 20h vs 44h, 44h vs 68h, 68h vs 92h, 20h vs 68h, 20h vs 92...
ORGANISM(S): Bos taurus 
2013-06-03 | E-GEOD-40916 | biostudies-arrayexpress
TranscriptomicsTranscriptional profiling of the luteinizing hormone surge in bovine granulosa cells (GC) during the pre-ovulation period comparing 2 h pre-LH GC, 6 h post-LH GC and 22 h post-LH GC
The LH surge induces panoply of events that are essential for ovulation and corpus luteum formation. The transcriptional responses to the LH surge of pre-ovulatory granulosa cells are complex and still poorly understood. In the present study, a genome wide bovine oligo array was used to determine ho...
ORGANISM(S): Bos taurus 
2010-10-15 | E-GEOD-23900 | biostudies-arrayexpress
TranscriptomicsDNA methylation analysis of bull semen according to peripubertal age (10 months vs. 16 months)
The DNA methylation pattern in spermatozoa of bulls at different age was investigated. Spermatozoa collected from 4 different bulls at 10, 12 and 16 month each. Genome-wide DNA methylation analysis was performed by micro array using EDMA platform. three condition experiment, 4 bulls, all collected ...
ORGANISM(S): Bos taurus 
2016-02-24 | E-GEOD-78231 | biostudies-arrayexpress
TranscriptomicsDNA methylation analysis of bull semen according to peripubertal age (12 months vs. 16 months)
The DNA methylation pattern in spermatozoa of bulls at different age was investigated. Spermatozoa collected from 4 different bulls at 10, 12 and 16 month each. Genome-wide DNA methylation analysis was performed by micro array using EDMA platform. three condition experiment, 4 bulls, all collected ...
ORGANISM(S): Bos taurus 
2016-02-24 | E-GEOD-78232 | biostudies-arrayexpress
TranscriptomicsGene expression analysis of bovine oocytes at optimal coasting time combined with GnRH antagonist during the no-FSH period
The objective of the study was to analyze the effect of a GnRH antagonist used during the FSH withdrawal period (coasting) on transcriptome changes in in vivo bovine oocytes at the GV stage. Oocytes were collected from super-stimulated animals after a coasting duration of 68hrs (optimal condition, c...
ORGANISM(S): Bos taurus 
2014-02-25 | E-GEOD-44664 | biostudies-arrayexpress
TranscriptomicsTranscriptome Analysis Of Bovine Expanded Blastocysts Produced In Vivo Vs In Vitro Sof Opu
Comparison of gene expression from expanded bovine blastocysts collected 7 days after fertilization and produced in vivo vs in vitro-SOF-OPU Two kinds of 7 days post fertilization bovine embryos, in-vivo 7 days blastocysts vs. In vitro 7 days blastocysts, Biological replicates: 4 in-vitro, 4 in-viv...
ORGANISM(S): Bos taurus 
2012-03-31 | E-GEOD-35025 | biostudies-arrayexpress
TranscriptomicsCellular and molecular characterization of the impact of laboratory setup on bovine in vitro embryo production
Comparison of gene expression from expanded bovine blastocysts collected 7 days after fertilization and produced in vitro in two different laboratories using the same protocol. Two different laboratories with same condition, site A blastocysts vs. Site B blastocysts, Biological replicates: 3 site A,...
ORGANISM(S): Bos taurus 
2012-03-31 | E-GEOD-34958 | biostudies-arrayexpress
TranscriptomicsCumulus cell gene expression associated with pre-ovulatory acquisition of developmental competence in bovine oocytes
This study investigated the cumulus cell (CC) transcriptomic changes during the oocyte developmental competence acquisition period. Six dairy cows were used for 24 oocyte collections and received FSH twice daily over 3 days, followed by FSH withdrawal for 20, 44, 68 and 92 h in four different oestro...
ORGANISM(S): Bos taurus 
2016-04-01 | E-GEOD-79760 | biostudies-arrayexpress
TranscriptomicsComparison of L-carnitine treated day 6 in vivo bovine embryos to controls, in both the Jersey and Holstein breeds.
The decreased rate of pregnancy obtained in cattle using frozen in vitro embryos compared to in vivo embryos has been associated with over-accumulation of intracellular lipid, which causes cell damage during cryopreservation. It is believed that the higher lipid content of blastomeres of bovine embr...
ORGANISM(S): Bos taurus 
2014-10-23 | E-GEOD-62595 | biostudies-arrayexpress
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