Male Sprague-Dawley rats were given NOS inhibitor L-NNA (500 mg/l water) for 4 days, 21 days or 21 days with Vitamin E in chow (0.7 g/kg BW/day) (4d-LNNA, 21d-LNNA and 21d-LNNA+VitE, respectively).
A time-course experiment was performed: HUVECs were exposed for 2, 4, 8 and 24 hours to (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate (DETA-NONOate) 250 uM.
HUVECs were exposed to 250 uM DETA-NONOate for 4 hours in the presence and absence of 10 uM of the guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo-[4,3,2]quinoxalin-1-one (ODQ). Control cells were exposed to vehicle (methanol 0.1%).
