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In this study, we have analyzed DNA methylation changes upon long-term culture and aging of MSC by using the HumanMethylation27 BeadChip assessing 27,578 unique CpG sites. Cells were taken from bone marrow aspirates from iliac crest (BM) of healthy donors or from the caput femoris (HIP) of elderly p...
ORGANISM(S): Homo sapiens 
In this study, we have analyzed DNA methylation changes upon aging of human dermal fibroblasts by using the HumanMethylation27 BeadChip assessing 27,578 unique CpG sites. Cells were isolated from skin samples donated by young (6-23 years) and elderly (60-73 years) patients undergoing surgical interv...
ORGANISM(S): Homo sapiens 
Transcription profiling of human mesenchymal cells (MSCs) after ex vivo expansion under culture conditions supporting extensive cell proliferation. Highly efficient cell expansion within short time (~40 days) and comparison of gene expression profiles from MSCs after primary seeding, defined as earl...
ORGANISM(S): Homo sapiens 
To determine gene expression changes during in vitro senescence of MSC we have analyzed differential expression of the corresponding early passage (P2) and senescent passage (PX). There were global changes in the gene expression profile that were reproducible in three independent donor samples. Expe...
ORGANISM(S): Homo sapiens 
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