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I infected 8 flasks of resting murine bone marrow derived macrophages with M. tuberculosis CDC1551. Individual samples of intracellular Mtb were collected at 2hr, and days 2,4,6,8,10,12, and 14 post-infection using the GTC (guanidine thiocyanate) method as previously published. A 2hr "no macr...
ORGANISM(S): Mycobacterium tuberculosis H37Rv 
Background: Conflicting results have been reported about the role of the two-component sensor and transcriptional regulator DosS/DosR, controlling the expression of the dormancy DosR regulon, for in vivo virulence of M. tuberculosis. Here, we have used a new approach to further analyze the relevance...
ORGANISM(S): Mycobacterium tuberculosis 

Pinus radiata seedlings, the most widely planted pine species in the world, were exposed to temperatures within a range mimicking future scenarios based on current models of heat increase. The short-term heat response in P. radiata was studied in detail by exploring the metabolo...

2023-08-14 | MTBLS4567 | MetaboLights
Large-scale identification of secreted and membrane-associated gene products using DNA microarrays. This study is described more fully in Diehn M et al.(2000) Nat Genet 25:58-62
ORGANISM(S): Homo sapiens 
mRNA expression data from next generation sequencing platforms is obtained in the form of counts per gene or exon. Counts are generally assumed to follow a Poisson distribution in which the variance is equal to the mean. However, it is common in observed count data with biological variation for over...
ORGANISM(S): Homo sapiens 
MATERIALS AND METHODS: The genomic effects of tumor-endothelial interactions in cancer are not yet well characterized. To study this interaction in breast cancer, we set up an ex vivo coculture model with human benign and malignant breast epithelial cells with endothelial cells to determine the asso...
ORGANISM(S): Homo sapiens 
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