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TranscriptomicsActin Cytoskeleton Integrates Auxin and Brassinosteroid Signaling in Plants.
We describe a new mutant allele of the ACTIN2 gene with enhanced actin dynamics, displaying a broad array of twisting and bending phenotypes that resemble BR-treated plants. Moreover, auxin transcriptional regulation is enhanced on the mutant background, supporting the idea that shaping actin filame...
ORGANISM(S): Arabidopsis thaliana 
2012-11-21 | E-GEOD-27077 | biostudies-arrayexpress
TranscriptomicsSpatiotemporal brassinosteroid signaling and antagonism with auxin control Arabidopsis root growth.
Analysis of brassinosteroid (BR) and auxin effects on gene expression in Arabidopsis roots. Our genomic results indicate that BR and auxin induce largely opposite gene expression responses in primary roots. RNA-Seq for 7-day-old Arabidopsis Col-0, dwf4, bri1-116, and bri1-116;bzr1-1D roots grown on ...
ORGANISM(S): Arabidopsis thaliana 
2015-04-30 | E-GEOD-52966 | biostudies-arrayexpress
TranscriptomicsAuxin stimulates brassinosteroid biosynthesis in Arabidopsis roots
We found that auxin stimulates gene expression of DWF4, which encodes a rate-dertermining step in brassinosteroid biosynthesis pathways. This increased gene expressioin subsequently led to elevation of the biosynthetic flux in Arabidopsis roots. To determine the list of genes that are regulated by a...
ORGANISM(S): Arabidopsis thaliana 
2011-02-15 | E-GEOD-12964 | biostudies-arrayexpress
GenomicsmiR394 modulates brassinosteroid signaling to regulate hypocotyl elongation in Arabidopsis
miR394 modulates brassinosteroid signaling to regulate hypocotyl elongation in Arabidopsis
PRJNA649706 | ENA
TranscriptomicsTranscription profiling of Arabidopsis Brassinosteroid mutant (bzr1-1D and det2) analysis
Brassinosteroid (BR) homeostasis and signaling are crucial for normal growth; and development of plants. BR signaling through cell-surface receptor; kinases and intracellular components leads to dephosphorylation and; accumulation of the nuclear protein BZR1. How BR signaling regulates gene; express...
ORGANISM(S): Arabidopsis thaliana 
2008-06-12 | E-GEOD-2696 | biostudies-arrayexpress
TranscriptomicsTranslatome analyses of opposing tissue-specific brassinosteroid signals orchestrating root meristem differentiation
The mechanisms ensuring balanced growth remain a critical question in developmental biology. In plants, this balance relies on spatiotemporal integration of hormonal signaling pathways, but the understanding of the precise contribution of each hormone is just beginning to take form. Brassinosteroid ...
ORGANISM(S): Arabidopsis thaliana 
2015-01-04 | E-GEOD-64410 | biostudies-arrayexpress
ProteomicsArabidopsis thaliana whole-proteome BIN2 kinase assay
Finely ground leaf tissue of wild-type columbia-0 ecotype was used. Protein extraction was performed in buffer-saturated phenol and high sucrose buffer. Extracted proteins were incubated with purified recombinant GST or GST-BIN2 kinase. Filter-assisted sample preparation (FASP) columns in prese...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-11-17 | MSV000086462 | MassIVE
GenomicsInteractions of ARF6 with PIF4, BZR1, and RGA integrate auxin signaling with environmental and other hormonal signals in Arabidopsis [ChIP-Seq]
Auxin is a major plant hormone for both development and environmental adaptation. Auxin responses are context dependent and highly modulated by light, temperature, the circadian clock, brassinosteroid, and gibberellin, but the underlying mechanisms remain unclear. Here, we show that auxin signaling ...
ORGANISM(S): Arabidopsis thaliana 
2014-06-03 | E-GEOD-51770 | biostudies-arrayexpress
TranscriptomicsInteractions of ARF6 with PIF4, BZR1, and RGA integrate auxin signaling with environmental and other hormonal signals in Arabidopsis [RNA-Seq]
Auxin is a major plant hormone for both development and environmental adaptation. Auxin responses are context dependent and highly modulated by light, temperature, the circadian clock, brassinosteroid, and gibberellin, but the underlying mechanisms remain unclear. Here, we show that auxin signaling ...
ORGANISM(S): Arabidopsis thaliana 
2014-06-03 | E-GEOD-51772 | biostudies-arrayexpress
ProteomicsBIN2 and RAPTOR1B mutants phosphoproteomic analysis
Finely ground leaf tissue was used. Extraction was done in 8M urea, precipitated with acetone and methanol. Filter-assisted sample preparation (FASP) columns were used to further purify proteins. Purified proteins were reduced with TCEP, alkylated with iodoacetamide and digested into peptides with ...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-11-17 | MSV000086460 | MassIVE
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