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TranscriptomicsThe genetic framework of the Drosophila piRNA pathway
The animal piRNA pathway is a small RNA silencing system that acts in gonads and protects the genome against the deleterious influence of transposons. A major bottleneck in the field is the lack of comprehensive knowledge of the factors and molecular processes that constitute this pathway. We conduc...
ORGANISM(S): Drosophila melanogaster 
2013-05-10 | E-GEOD-45894 | biostudies-arrayexpress
GenomicsDrosophila Gtsf1 is an essential component of the Piwi mediated transcriptional silencing complex
The PIWI interacting RNA pathway is a small RNA silencing system that keeps selfish genetic elements such as transposons under control in animal gonads. Several lines of evidence indicate that nuclear PIWI family proteins guide transcriptional silencing of their targets, yet the composition of the u...
ORGANISM(S): Drosophila melanogaster 
2013-09-06 | E-GEOD-48317 | biostudies-arrayexpress
TranscriptomicsTranscriptional silencing of transposable elements by Piwi and its impact on chromatin and gene expression
Eukaryotic genomes are colonized by transposable elements whose uncontrolled activity results in genomic instability. The piRNA pathway silences transposons in animal gonads, yet how this is achieved molecularly remains controversial. We assign an essential role to the HMG protein Maelstrom in the p...
ORGANISM(S): Drosophila melanogaster 
2012-11-26 | E-GEOD-41729 | biostudies-arrayexpress
TranscriptomicsHigh throughput sequencing of Piwi bound piRNAs from Drosophila ovaries in which key factors for primary piRNA biogenesis in somatic support cells were knocked down using RNAi
In Drosophila, PIWI proteins and bound PIWI interacting RNAs (piRNAs) form the core of a small RNA mediated defense system against selfish genetic elements. Within germline cells piRNAs are processed from piRNA clusters and transposons to be loaded into Piwi/Aubergine/AGO3 and a subset of piRNAs und...
ORGANISM(S): Drosophila melanogaster 
2010-10-03 | E-GEOD-23560 | biostudies-arrayexpress
TranscriptomicsNovel piRNA Pathway Components Identified Among the Class of TUDOR Domain Containing Proteins
PIWI proteins and their bound piRNAs form the core of a gonad specific small RNA silencing pathway in animals that protects the genome against the deleterious activity of transposable elements. Recent studies linked the piRNA pathway to TUDOR biology, where TUDOR domains of various proteins recogniz...
ORGANISM(S): Drosophila melanogaster 
2011-08-16 | E-GEOD-30955 | biostudies-arrayexpress
TranscriptomicspiRNA-guided slicing of transposon transcripts enforces their transcriptional silencing via specifying the nuclear piRNA repertoire
PIWI-clade Argonaute proteins silence transposon expression in animal gonads. Their target specificity is defined by bound ~23-30nt piRNAs that are processed from single-stranded precursor transcripts via two distinct pathways. Primary piRNAs are defined by the endo-nuclease Zucchini, while biogenes...
ORGANISM(S): Drosophila melanogaster 
2015-08-31 | E-GEOD-71775 | biostudies-arrayexpress
TranscriptomicsThe Rhino-Deadlock-Cutoff complex licenses non-canonical transcription of dual-strand piRNA clusters in Drosophila
Argonaute proteins of the PIWI clade are central to transposon silencing in animal gonads. Their target specificity is defined by 22-30nt PIWI interacting RNAs (piRNAs), which mostly originate from discrete genomic loci termed piRNA clusters. Here we show that the RDC complex composed of Rhino, Dead...
ORGANISM(S): Drosophila melanogaster 
2014-06-08 | E-GEOD-55824 | biostudies-arrayexpress
ProteomicsA heterochromatin-dependent transcription machinery drives piRNA expression
Nuclear small RNA pathways safeguard genome integrity by establishing transcription-repressing heterochromatin at transposable elements. This inevitably also targets the transposon-rich source loci of the small RNAs themselves. How small RNA source loci are efficiently transcribed while transposon p...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2017-08-07 | PXD005026 | Pride
Transcription Drosophila Ovary Pirna 
ProteomicsA heterochromatin specific RNA export pathway facilitates piRNA production
PIWI-interacting RNAs (piRNAs), a class of small RNAs that guide transposon silencing in animals, are processed in the cytoplasm from RNA Polymerase II transcripts. How piRNA precursors, which often lack RNA maturation signatures and thereby violate quality control checkpoints, achieve nuclear expor...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-03-29 | PXD012717 | Pride
Export Drosophila Ovary Pirna 
TranscriptomicsAn epigenetic role for maternally inherited piRNAs in transposon silencing
In plants and mammals, small RNAs indirectly mediate epigenetic inheritance by specifying cytosine methylation. Here, we find that small RNAs themselves serve as vectors for epigenetic information. Crosses between Drosophila strains that differ in the presence of a particular transposon can pr...
ORGANISM(S): Drosophila melanogaster 
2008-11-28 | E-GEOD-13081 | biostudies-arrayexpress
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