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TranscriptomicsAn Ancient Transcription Factor Initiates the Burst of piRNA Production During Early Meiosis in Mouse Testes (smallRNA-Seq)
Animal germ cells produce PIWI-interacting RNAs (piRNAs), small silencing RNAs that suppress transposons and enable gamete maturation. Mammalian transposon-silencing piRNAs accumulate early in spermatogenesis, whereas pachytene piRNAs are produced later during post-natal spermatogenesis and account ...
ORGANISM(S): Gallus gallus 
2013-03-27 | E-GEOD-45049 | biostudies-arrayexpress
TranscriptomicspiRNA production requires heterochromatin formation in Drosophila
Here, we analyzed two small RNA libraries derived from ovarian tissue mutant for either the Drosophila SETDB1 gene, or the Bam gene. Here we show that deposition of histone 3 lysine 9 by the methyltransferase dSETDB1 (egg) is required for piRNA cluster transcription. In the absence of dSETDB1, clust...
ORGANISM(S): Drosophila melanogaster 
2011-08-15 | E-GEOD-30086 | biostudies-arrayexpress
TranscriptomicsSpecialized piRNA pathways act in germline and somatic tissues of the Drosophila ovary
Here, we analyzed nine Drosophila piRNA pathway mutants for their impacts on both small RNA populations and the sub-cellular localization patterns of Piwi proteins. We find that distinct piRNA pathways with differing components function in ovarian germ and somatic cells. Keywords: Epigenetics 20 li...
ORGANISM(S): Drosophila melanogaster 
2009-04-29 | E-GEOD-15186 | biostudies-arrayexpress
TranscriptomicsVreteno, a gonad-specific protein, is essential for germline development and primary piRNA biogenesis in Drosophila.
Here, we analyzed small RNA libraries derived from ovarian tissues heterozygous or mutant for the Tudor gene, Vreteno. In the absence of vret, Piwi-bound piRNAs are lost without changes in piRNA precursor transcript production, supporting a role for Vret in primary piRNA biogenesis. In the germline,...
ORGANISM(S): Drosophila melanogaster 
2011-08-15 | E-GEOD-30088 | biostudies-arrayexpress
TranscriptomicsHigh throughput sequencing of Piwi bound piRNAs from Drosophila ovaries in which key factors for primary piRNA biogenesis in somatic support cells were knocked down using RNAi
In Drosophila, PIWI proteins and bound PIWI interacting RNAs (piRNAs) form the core of a small RNA mediated defense system against selfish genetic elements. Within germline cells piRNAs are processed from piRNA clusters and transposons to be loaded into Piwi/Aubergine/AGO3 and a subset of piRNAs und...
ORGANISM(S): Drosophila melanogaster 
2010-10-03 | E-GEOD-23560 | biostudies-arrayexpress
TranscriptomicsThe Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline
In a broad range of organisms, Piwi-interacting RNAs (piRNAs) have emerged as core components of a surveillance system that protects the genome by silencing transposable and repetitive elements. A vast proportion of piRNAs is produced from discrete genomic loci, termed piRNA clusters. The molecular ...
ORGANISM(S): Drosophila melanogaster 
2013-06-07 | E-GEOD-47738 | biostudies-arrayexpress
GenomicspiRNA production requires heterochromatin formation in Drosophila
Here, we analyzed H3K9me3 derived from wild type ovarian tissue. Here we show that deposition of histone 3 lysine 9 by the methyltransferase dSETDB1 (egg) is required for piRNA cluster transcription. In the absence of dSETDB1, cluster precursor transcription collapses in germline and somatic gonadal...
ORGANISM(S): Drosophila melanogaster 
2011-09-08 | E-GEOD-30157 | biostudies-arrayexpress
TranscriptomicsConserved generation of short products at piRNA loci
We analyzed small RNAs from three mammalian species, and found that in all these species piRNA-directed targeting is accompanied by the generation of short sequences that have a very precisely defined length and a specific spatial relationship with the guide piRNAs. small RNA-seq of testes lysate (b...
ORGANISM(S): Mus musculus 
2011-03-07 | E-GEOD-26160 | biostudies-arrayexpress
TranscriptomicsWidespread Expression of piRNA-like Molecules in Somatic Tissues
Piwi-interacting RNA (piRNA) are small RNA abundant in the germline across animal species. In fruit flies and mice, piRNA were implicated in maintenance of genomic integrity by transposable elements silencing. Outside of the germline, piRNA have only been found in Drosophila ovarian follicle cells. ...
ORGANISM(S): Mus musculus 
2011-06-01 | E-GEOD-27369 | biostudies-arrayexpress
TranscriptomicsUAP56 couples piRNA clusters to the perinuclear transposon silencing machinery [tiling array]
The transposon silencing piRNAs are produced from precursors that are encoded by heterochromatic clusters and processed in the perinuclear nuage. We show that the Drosophila nuclear DEAD box protein UAP56, previously implicated in mRNA splicing and nuclear export, co-localizes with the cluster-assoc...
ORGANISM(S): Drosophila melanogaster 
2012-11-28 | E-GEOD-35636 | biostudies-arrayexpress
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