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TranscriptomicsmRNA-Seq from control cells and two clones of Flp-In-T-REx-293 cells modified via CRISPR-Cas9 to knockout SMG7 in combination to knockdowns of control, SMG5, and SMG6
Mature mRNAs undergo quality control during translation that may lead to RNA degradation by triggering the nonsense mediated decay (NMD) pathway. Aberrant translation due to features such as the presence of a premature stop codon downstream on an exon-exon junction or an intron in the 3'UTR activate...
ORGANISM(S): Homo sapiens 
2021-05-07 | E-MTAB-9330 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of Flp-In T-REx 293, two clones of CRISPR-Cas9 mediated knockout of CASC3 and further CASC3 depletion via small interfering RNAs
We report two knockouts of the CASC3 (Barentsz, MLN51) gene and further depletion of gene expression with small interfering RNAs (siRNAs). CASC3 is a component of the exon-junction complex (EJC) which is deposited upstream of splice junctions on the mRNA. The EJC core and peripheral interacting fact...
ORGANISM(S): Homo sapiens 
2020-07-03 | E-MTAB-8461 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of control, RNPS1 knockdown and rescue in HeLa cells
We report the knockdown and rescue of RNPS1, a component of the ASAP and PSAP complexes and, hence, an auxiliary component of the exon junction complex. The studied conditions show an extensive change in alternative splicing and the cell's transcriptome. For the knockdown, HeLa cells were transfect...
ORGANISM(S): Homo sapiens 
2018-11-01 | E-MTAB-6564 | biostudies-arrayexpress
TranscriptomicsHeart RNA-Seq of cardiomyocyte-specific and inducible Prmt5 knockout mouse model, AVV9-ACADVL rescue
Our study introduces a novel animal model featuring a cardiomyocyte-specific, inducible knockout of the Prmt5. The primary aim of this model is to investigate the regulatory mechanisms of PRMT5, the main representative of type II PRMTs. We found that PRMT5 controls Acadvl mRNA splicing, and that dis...
ORGANISM(S): Mus musculus 
2026-05-15 | E-MTAB-14396 | biostudies-arrayexpress
TranscriptomicsHeart RNA-Seq of cardiomyocyte-specific and inducible Prmt5 knockout mouse model, nuclear sequencing without treatment
Our study introduces a novel animal model featuring a cardiomyocyte-specific, inducible knockout of the Prmt5. The primary aim of this model is to investigate the regulatory mechanisms of PRMT5, the main representative of type II PRMTs. We found that PRMT5 controls Acadvl mRNA splicing, and that dis...
ORGANISM(S): Mus musculus 
2026-05-15 | E-MTAB-14401 | biostudies-arrayexpress
TranscriptomicsEffect of high-dose epinephrine on myocardial gene set expression
Male and female mice were injected with NaCl vs epinephrine to induce acute paradoxical reversible heart failure akin to Takotsubo Syndrome with subsequent RNASeq analysis of left ventricular tissue.
ORGANISM(S): Mus musculus 
2023-05-31 | E-MTAB-13031 | biostudies-arrayexpress
TranscriptomicsHeart RNA-Seq of cardiomyocyte-specific and inducible Prmt5 knockout mouse model
Our study introduces a novel animal model featuring a cardiomyocyte-specific, inducible knockout of the Prmt5. The primary aim of this model is to investigate the regulatory mechanisms of PRMT5, the main representative of type II PRMTs. We found that PRMT5 controls Acadvl mRNA splicing and that disr...
ORGANISM(S): Mus musculus 
2026-05-15 | E-MTAB-13594 | biostudies-arrayexpress
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