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ProteomicsCharacterization of disulfide linkages in proteins by 193 nm ultraviolet photodissociation (UVPD)
Inter-linked disulfide bonds connecting peptide chains are homolytically cleaved with 193 nm ultraviolet photodissociation (UVPD). Analysis of insulin demonstrates the ability for UVPD to cleave multiple disulfide bonds and provide sequence coverage of multiple peptide chains in the same MS/MS event...
ORGANISM(S): Homo Sapiens (human) Gallus Gallus Bos Taurus 
2018-04-28 | PXD009460 | JPOST Repository
ProteomicsApplication of 193 nm UVPD for phosphoproteomic analysis of HeLa and HCC70
Application of 193 nm ultraviolet photodissociation (UVPD) for phosphoproteomics analysis of IMAC enriched HeLa and HCC70 lysates. HCC70 cells were either maintained in full media or serum starved for 6 hours prior to lysis. Each sample was analyzed by three UVPD and three HCD technical replicates u...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-04-04 | MSV000079626 | MassIVE
ProteomicsEnhanced Characterization of Lysine-Linked Antibody Drug Conjugates Enabled by Middle-Down Mass Spectrometry
HCD Triggered UVPD and EThcD files for characterization of trastuzumab-emtansine (T-DM1)
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-08-03 | MSV000092585 | MassIVE
ProteomicsAMCA chromophore tagging and 351 nm ultraviolet photodissociation enables de novo sequencing of E. coli proteome
Demonstration of novel experimental and computational pipeline for high performance de novo peptide sequencing. E. coli whole cell lysate is carbamylated to block lysine side chains, digested with trypsin (now active only at Arg residues), and N-terminally tagged with the chromophore AMCA. Three tec...
ORGANISM(S): Escherichia Coli (ncbitaxon:562) 
2016-03-10 | MSV000079571 | MassIVE
ProteomicsComplementary CID and 351 nm UVPD tandem mass spectra enable effective de novo sequencing
This dataset accompanies a publication in which we present a strategy for acquisition and effective de novo peptide sequencing of complementary CID and 351 nm ultraviolet photodissociation (UVPD) MS/MS pairs. E. coli whole cell lysate is carbamylated to block lysine side chains, digested with trypsi...
ORGANISM(S): Escherichia Coli (ncbitaxon:562) 
2017-03-06 | MSV000080603 | MassIVE
ProteomicsCharacterization of HLA-A*02:01 MHC Immunopeptide Antigens Enhanced by Ultraviolet Photodissociation Mass Spectrometry
Triplicate results for 193 nm UVPD, HCD, and EThcD of a mixture of 157 synthetic peptide mixture, and 193 nm UVPD, HCD, and EThcD of BB7.2 and W6/32 immunoprecipitations
ORGANISM(S): Homo Sapiens (human) 
2021-08-13 | PXD027830 | JPOST Repository
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