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TranscriptomicsTranscription profiling of wild type, p53 Ser389 phosphorylation site point mutant and p53 knock-out mouse embryonic fibroblasts after exposure to UV radiation for different lengths of time
Comparison of responses of mouse wild-type, p53 Ser389 phosphorylation site point mutant and full knock-out mutant MEF cells to UV-C-radiation in time
ORGANISM(S): Mus musculus 
2008-06-01 | E-MEXP-1397 | biostudies-arrayexpress
TranscriptomicsRNA isolation from mouse skin biopies
Study to optimize our protocol for isolating RNA from skin biopsies from (hairless) SKH mice using different-diameter biopsy punches. Some mice were also treated with UVB radiation to check its effect on RNA yield. 4 mice total: 2 were irradiated with 300J/m2 UVB and 2 were non-irradiated. Post-mor...
ORGANISM(S): Mus musculus 
2011-10-04 | E-GEOD-28463 | biostudies-arrayexpress
TranscriptomicsSingle tip transcriptomics of neighboring hyphae of Aspergillus niger
Mycelia of filamentous fungi explore new substrates by means of hyphae that extend from the periphery of the colony. Previously, it has been shown by immuno-labelling, reporter studies and in situ hybridization that these exploring hyphae are heterogenic with respect to protein secretion and transcr...
ORGANISM(S): Aspergillus niger 
2011-05-17 | E-GEOD-25497 | biostudies-arrayexpress
TranscriptomicsEvaluation of ribonucleic acid amplification protocols for human oocyte transcriptome analysis
Objective: To develop a reliable, reproducible, and sensitive method for investigating gene-expression profiles from individual human oocytes. Setting: University medical center and university microarray laboratory. Patient(s): Couples undergoing intracytoplasmic sperm injection treatment were aske...
ORGANISM(S): Homo sapiens 
2015-12-31 | E-GEOD-48141 | biostudies-arrayexpress
TranscriptomicsUV-C dose and time range finding study on Mouse Embryonic Fibroblasts (MEFs)
In molecular biology, the design of mechanistic experiments has to be optimized by considering statistical and biological principles. In contrast to statistical principles, biological principles of experimental design are not universally formulated. In an attempt to pinpoint generally acceptable rul...
ORGANISM(S): Mus musculus 
2014-09-01 | E-GEOD-50930 | biostudies-arrayexpress
TranscriptomicsConfounding factors in the analysis of the transcriptome response to UV exposure in skin of wild-type and human p53-variant mice
An in-vivo experiment with UV-B exposure of the skin of nude, male mice, with and without human-derived p53 variants in their genome to investigate the cellular responses upon UV-induced DNA damage. Essentially, 4 replicate mice were used for each level of UV-exposure. For each mouse a biopsy of the...
ORGANISM(S): Mus musculus 
2016-03-01 | E-GEOD-63044 | biostudies-arrayexpress
TranscriptomicsUV-B dose and time range finding study on mouse biopsy skin samples
In molecular biology, the design of mechanistic experiments has to be optimized by considering statistical and biological principles. In contrast to statistical principles, biological principles of experimental design are not universally formulated. In an attempt to pinpoint generally acceptable rul...
ORGANISM(S): Mus musculus 
2014-09-01 | E-GEOD-51348 | biostudies-arrayexpress
TranscriptomicsEffect of multiple longitudinal biopsy sampling in individual mice
Small scale study to asses the effects of taking multiple skinbiopts from individual mice in time. Results showed no influence on expression levels in time from older samplings on newer samplings. skin biopts from 4 individual mice in taken at 6 points in time
ORGANISM(S): Mus musculus 
2014-09-01 | E-GEOD-51347 | biostudies-arrayexpress
TranscriptomicsA novel aCGH method for non-model organisms for assessing genomes and de-novo transcriptome sequencing reads (Danio rerio)
Genomes and transcriptomes of non-model organisms can be analyzed using next-generation sequencing technologies, but de-novo sequencing and annotating a full eukaryotic genome is still challenging. So, -omics experimentation with non-model organisms requires a suite of technologies to obtain reliabl...
ORGANISM(S): Danio rerio 
2014-05-19 | E-GEOD-52121 | biostudies-arrayexpress
TranscriptomicsA novel aCGH method for non-model organisms for assessing genomes and de-novo transcriptome sequencing reads (Staphylococcus aureus)
Genomes and transcriptomes of non-model organisms can be analyzed using next-generation sequencing technologies, but de-novo sequencing and annotating a full eukaryotic genome is still challenging. So, -omics experimentation with non-model organisms requires a suite of technologies to obtain reliabl...
ORGANISM(S): Staphylococcus aureus 
2014-05-19 | E-GEOD-51148 | biostudies-arrayexpress
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