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ProteomicsTET1 interactome in mESCs (via ChIP-MS)
Precise regulation of DNA methylation in mammals is critical for genome stability and epigenetic regulation. The discovery of the ten-eleven translocation (TET) proteins catalyzing the oxidation from 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcyto...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-31 | PXD028850 | Pride
Epigenetics Tet1 Mouse embryonic stem cells Dna methylation 
ProteomicsDistinct and stage-specific contributions of TET1 and TET2 to stepwise cytosine oxidation in the transition from naive to primed pluripotency
Cytosine DNA bases can be methylated by DNA methyltransferases and subsequently oxidized by TET proteins. The resulting 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC) are considered demethylation intermediates as well as stable epigenetic marks. To dissect the ...
ORGANISM(S): Mus musculus (Mouse) 
2020-07-24 | PXD019514 | Pride
Epigenetics Epiblast-like cells Mouse embryonic stem cells Dna methylation 
ProteomicsDissection of TET1 catalytic and non-catalytic functions reveals DNA demethylation independent gene and ERV regulation
Surveillance of DNA methylation in mammals is critical for genome stability and epigenetic regulation. The discovery of the ten-eleven translocation (TET) proteins catalyzing the oxidation from 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-11 | PXD028566 | Pride
Methylation Acetylation Tet1 Histone ptms 
TranscriptomicsTETs controls maintenance DNA methylation via the regulation of Dppa3
Dissection of catalytic and non-catalytic functions of Tet1 and Tet2. Transcriptome profiling of wild type, Tet1 catalytic mutants, Tet2 and Tet1/2 catalytic mutant catalytic during EpiLC differentiation.
ORGANISM(S): Mus musculus 
2020-10-05 | E-MTAB-6797 | biostudies-arrayexpress
TranscriptomicsHP1β dependent phase separation regulates embryonic stem cells exit from naïve pluripotent state
Liquid-liquid phase separation has drawn great attention as a physical process that mediates the formation of membraneless organelles in cells to coordinate biological reactions in space and time. Previously, it was proposed that the formation of phase-separated droplets is a unique property of HP1α...
ORGANISM(S): Mus musculus 
2022-05-01 | E-MTAB-8329 | biostudies-arrayexpress
TranscriptomicsHigh-throughput CRISPR screen to identify factors involved in DNA methylation maintenance
Mouse embryonic stem cells have been modified with a DNA methylation reporter based on the Sprn promoter system published in Stelzer et al. (Cell, 2015). This reporter allows selection by Hygromycin as well as mScarlet expression. The cells were infected with a Lentiviral genome-wide CRISPR/Cas libr...
ORGANISM(S): Mus musculus 
2022-01-01 | E-MTAB-9126 | biostudies-arrayexpress
GenomicsLINE-1 DNA methylation levels in DPPA3 KO overexpressing DPPA3 mutants
DPPA3 mutants were overexpressed using a doxycycline system to identify regions in DPPA3 necessary for DNA methylation maintenance suppression
ORGANISM(S): Mus musculus 
2020-10-22 | E-MTAB-9653 | biostudies-arrayexpress
GenomicsAmplicon bisulfite sequencing of LINE-1 elements in TET catalytic mutants and DPPA3 KO ESCs rescued by doxycylin induced overexpression
Tet1, Tet2 and Tet1/Tet2 catalytic mutants as well as DPPA3 KO ESCs harboring a doxycyclin inducible Dppa3 transgene were induced for several days to monitor LINE-1 methylation levels.
ORGANISM(S): Mus musculus 
2020-10-23 | E-MTAB-9654 | biostudies-arrayexpress
TranscriptomicsRNA-seq of TET1 knockout, catalytic mutant and wild type mESCs
Investigation of TET1 non-catalytic functions in mESCs cultured in serum + LIF (primed)
ORGANISM(S): Mus musculus 
2022-07-29 | E-MTAB-10937 | biostudies-arrayexpress
GenomicsDNA methylation profiling to investigate how Tet1 controls maintenance DNA methylation via the regulation of Dppa3 in ESCs
DNA methylation profiling using RRBS in wild type and Dppa3 knockout ESCs.
ORGANISM(S): Mus musculus 
2020-09-30 | E-MTAB-6800 | biostudies-arrayexpress
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