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TranscriptomicsTransposon random mutagenesis of Mycoplasma pneumoniae M129. Samples sequenced for passage numbers 1, 2, 3, 4, 6, 8 and 10, two biological replicates per passage and two technical replicates for passages 2-4.
Controlled dataset covering multiple passage conditions, from the genome-reduced bacterium Mycoplasma pneumoniae, to test and validate two new software tools: FASTQINS and ANUBIS. These computational tools allow the generation of insertion profile datasets from raw transposon sequencing data, detect...
ORGANISM(S): Mycoplasma pneumoniae M129 
2020-08-28 | E-MTAB-8918 | biostudies-arrayexpress
ProteomicsLabel-free shotgun proteomics of Mycoplasma pneumoniae grown in serum-free medium
We developed a serum and animal component-free medium capable of supporting robust growth of the genome-reduced bacterium Mycoplasma pneumoniae. Here, we analyzed the proteome of the wild-type strain grown in serum-free vB13 medium compared to rich medium (Hayflick medium).
ORGANISM(S): Mycoplasma pneumoniae (strain ATCC 29342 / M129) Bacteria 
2023-04-05 | PXD037431 | Pride
Mycoplasma pneumoniae Serum-free medium 
GenomicsTransposon random mutagenesis with MPN625 intrinsic terminator of Mycoplasma pneumoniae M129 (lab strain). Samples sequenced for passage numbers 1, 2, 3, 4, 6, 8 and 10, two biological replicates per passage and two technical replicates for passages 2-4
Transposon random mutagenesis to randomly insert the intrinsic terminator associated to the gene mpn625 from Mycoplasma pneumoniae M129. Different passage conditions (each passage equivalent to 10 cell division) are collected including 1,2,3,4,6,8, and 10 passages. This dataset is complementary to E...
ORGANISM(S): Mycoplasmoides pneumoniae 
2025-01-01 | E-MTAB-14533 | biostudies-arrayexpress
TranscriptomicsRNA-seq differential expression analysis of tmRNA (ssrA) mutants in the genome-reduced bacterium Mycoplasma pneumoniae
We used a combination of genetic and proteomic approaches to characterize tmRNA (ssrA) activity in the genome-reduced bacterium Mycoplasma pneumoniae. For this, we generated tmRNA mutants encoding a tag resistant to proteolysis. Endogenous protein tagging by the mutant tmRNA gene (ssrAmk) was then e...
ORGANISM(S): Mycoplasma pneumoniae 
2021-08-09 | E-MTAB-10111 | biostudies-arrayexpress
TranscriptomicsRNA-seq of Lon and FtsH conditional mutants of Mycoplasma pneumoniae under depleting and inducing conditions
Using Mycoplasma pneumoniae as a model organism, we conditionally depleted the two essential ATP-dependent proteases (Lon and FtsH) of this bacterium, by engineering three strains carrying a Lon and/or FtsH inducible expression locus. An integrative comparative study combining label-free shotgun pro...
ORGANISM(S): Mycoplasma pneumoniae M129 
2020-11-10 | E-MTAB-8537 | biostudies-arrayexpress
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