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TranscriptomicsThe transcriptome of TRPV1-positive sensory neurons revealed by subgroup-elimination transcriptomics
We developed an approach to rapidly eliminate the subgroup of sensory neurons expressing the heat-gated cation channel TRPV1 from dissociated rat sensory ganglia using agonist treatment followed by density centrifugation. To identify transcripts predomintly expressed in TRPV1-positive neurons, we co...
ORGANISM(S): Rattus norvegicus 
2015-05-12 | E-GEOD-59727 | biostudies-arrayexpress
GenomicsGlucocorticoid receptor profiling (ChIP-seq) in U2OS-GR cells 24 hours after hormone washout
We performed ChIP-seq targeting the glucocorticoid receptor (GR) in the U2OS-GR cell line. The cell line is derived from U2OS ATTC:HTB-96 and stably transfected with an expression construct for rat GR. The cells were treated with 100 nM dexamethasone for 4 hours, washed 2x with PBS and cultured in h...
ORGANISM(S): Homo sapiens 
2021-05-03 | E-MTAB-9914 | biostudies-arrayexpress
UnknownSubgroup-elimination transcriptomics identifies signaling proteins that define subclasses of TRPV1-positive neurons and a novel paracrine circuit.
Not available
S-EPMC4281118 | biostudies-literature
ProteomicsIntegrative multi-omics analysis of growth plate regulation underlying body size in miniature pigs
Body size represents a complex phenotype driven by genetic variation and epigenetic regulation, with the molecular processes underlying this trait remaining a central challenge to disentangle. To elucidate these fundamental mechanisms, we applied a multi-omics approach to investigate the regulatory ...
ORGANISM(S): Sus scrofa (Pig) 
2026-06-11 | PXD069390 | Pride
Miniaturization Growth Miniature pigs Functional genomics Multi-omics Gene regulation Body size Growth plates 
UnknownHLA I immunopeptidome of synthetic long peptide pulsed human dendritic cells for therapeutic vaccine design.
Not available
S-EPMC11742953 | biostudies-literature
UnknownEnhanced receptor-clathrin interactions induced by N-glycan-mediated membrane micropatterning.
Not available
S-EPMC4121791 | biostudies-literature
UnknownProteome Based Construction of the Lymphocyte Function-Associated Antigen 1 (LFA-1) Interactome in Human Dendritic Cells.
Not available
S-EPMC4758637 | biostudies-literature
TranscriptomicsTotal RNA-seq in the A549 cell line after glucocorticoid re-induction
We performed total RNA-seq in the A549 cell line. Cells were treated with either 100 nM dexamethasone or 0.1% ethanol. After 4 hours, cells were washed 2x with PBS and cultured in hormone-free medium for 24 hours, after which cells were treated again for 4 hours with either 100 nM dexamethasone or 0...
ORGANISM(S): Homo sapiens 
2021-05-03 | E-MTAB-9923 | biostudies-arrayexpress
TranscriptomicsStudying changes in chromatin accessibility in A549 cells by ATAC-seq after glucocorticoid re-induction
We performed ATAC-seq in the A549 cell line. Cells were treated with either 100 nM dexamethasone or 0.1% ethanol. After 4 hours, cells were washed 2x with PBS and cultured in hormone-free medium for 24 hours, after which cells were treated again for 4 hours with either 100 nM dexamethasone or 0.1% e...
ORGANISM(S): Homo sapiens 
2021-05-03 | E-MTAB-9912 | biostudies-arrayexpress
TranscriptomicsStudying changes in chromatin accessibility in U2OS-GR cells by ATAC-seq directly after 4-hour glucocorticoid treatment and following a 24-hour hormone washout
We performed ATAC-seq in the U2OS-GR cell line. The cell line is derived from U2OS ATTC:HTB-96 and stably transfected with an expression construct for rat GR. Cells were treated with dexamethasone (100 nM) or vehicle (ethanol) for 4 hours and either harvested immediately or washed 2x with PBS and su...
ORGANISM(S): Homo sapiens 
2021-05-03 | E-MTAB-9909 | biostudies-arrayexpress
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