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GenomicsFlexible chromatin loops in the VH gene region of the Igh locus facilitate the generation of a diverse antibody repertoire
The immunoglobulin heavy-chain (Igh) locus undergoes large-scale contraction in pro-B cells, which facilitates VH-DJH recombination by juxtaposing distal VH genes next to the DJH- rearranged gene segment in the proximal Igh domain. By high-resolution mapping of long-range interactions, we now demons...
ORGANISM(S): Mus musculus 
2013-09-30 | E-GEOD-43008 | biostudies-arrayexpress
TranscriptomicsChip-chip from pro-B cells with H3K4me3, H3K4me2 and H3K9ac
The transcription factor Pax5 represses B-lineage-inappropriate genes and activates B-cell-specific genes in B-lymphocytes. Here we have identified 170 novel Pax5-activated genes. Conditional mutagenesis demonstrated that the Pax5-regulated genes require continuous Pax5 activity for normal expressio...
ORGANISM(S): Mus musculus 
2007-08-23 | E-GEOD-8472 | biostudies-arrayexpress
TranscriptomicsIGH analysis in pro-B cells with H3K4me3, H3K4me2 and H3K9ac
STAT5 and IL-7 signaling are thought to control B-lymphopoiesis by regulating key transcription factor genes and activating VH gene segments at the Igh locus. Using conditional mutagenesis, we demonstrate that transgenic Bcl2 expression rescued the development of Stat5-deleted pro-B cells by compens...
ORGANISM(S): Mus musculus 
2010-07-28 | E-GEOD-18278 | biostudies-arrayexpress
TranscriptomicsChip-chip from pro-B cells with H3K4me3, H3K4me2 and H3K9ac
The transcription factor Pax5 represses B-lineage-inappropriate genes and activates B-cell-specific genes in B-lymphocytes. Here we have identified 170 novel Pax5-activated genes. Conditional mutagenesis demonstrated that the Pax5-regulated genes require continuous Pax5 activity for normal expressio...
ORGANISM(S): Mus musculus 
2010-05-20 | E-GEOD-15145 | biostudies-arrayexpress
GenomicsIGH analysis in pro-B cells with H3K4me3, H3K4me2, H3K9ac, H3K27me3, Pax5 and CTCF [ChIP-Seq]
VH-DJH recombination of the immunoglobulin heavy-chain (Igh) locus is temporally and spatially controlled during early B-cell development, and yet no regulatory elements other than the VH gene promoters have been identified throughout the entire 2.5-Mb VH gene cluster. Here we discovered novel regul...
ORGANISM(S): Mus musculus 
2011-02-11 | E-GEOD-27214 | biostudies-arrayexpress
ProteomicsEssential role of the Pax5 C-terminal domain in controlling B cell commitment and development
Pax5 controls B-cell commitment, development and immunity by repressing B-lineage-inappropriate genes and activating B-cell-specific genes. In addition to the N-terminal DNA-binding paired domain, Pax5 contains a conserved octapeptide, partial homeodomain and C-terminal sequences with transactivatin...
ORGANISM(S): Mus musculus (Mouse) 
2023-08-24 | PXD040424 | Pride
Co-repressors C-terminal regulatory domains Gene activation Co-activators Pax5 Gene repression 
TranscriptomicsThe transcription factor Pax5 regulates its target genes by recruiting chromatin-modifying proteins in committed B cells
Pax5 is a critical regulator of B cell commitment. Here we identified direct Pax5 target genes by streptavidin-mediated ChIP-chip analysis of pro-B cells expressing in vivo biotinylated Pax5. By binding to promoters and enhancers, Pax5 directly regulates the expression of multiple transcription fact...
ORGANISM(S): Mus musculus 
2012-03-29 | E-GEOD-28373 | biostudies-arrayexpress
GenomicsEssential role of EBF1 in B cell immunity by controlling the generation and function of distinct mature B cell types
The transcription factor EBF1 is essential for lineage specification in early B cell development. Here we demonstrate by conditional mutagenesis that EBF1 was required for B cell commitment, pro-B cell development and subsequent transition to pre-B cells. Later in B cell development, EBF1 was essent...
ORGANISM(S): Mus musculus 
2012-03-20 | E-GEOD-35857 | biostudies-arrayexpress
GenomicsThe B cell identity factor Pax5 regulates distinct transcriptional programs in early and late B lymphopoiesis
Pax5 controls the identity and development of B cells by repressing lineage-inappropriate genes and activating B-cell-specific genes. Here, we used genome-wide approaches to identify Pax5 target genes in pro-B and mature B cells. In these cell types, Pax5 bound to 40% of the cis- regulatory elements...
ORGANISM(S): Mus musculus 
2012-06-14 | E-GEOD-38046 | biostudies-arrayexpress
TranscriptomicsThe transcription factor GATA3 controls cell fate and maintenance of type 2 innate lymphoid cells
The microarray was designed in order to detect transcriptional similarities/differences between innate immune cells (ILCs) in the lamina propria (N;L;R) and a putative precursor cell type detected in the bone marrow (P) of untreated mice in steady-state.
ORGANISM(S): Mus musculus 
2012-10-18 | E-MEXP-3743 | biostudies-arrayexpress
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