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GenomicsHigh resolution transcriptome-wide RNA cytosine methylome of Mouse Embryonic Fibroblasts
We report high resolution transcriptome-wide RNA cytosine methylome of Mouse Embryonic Fibroblasts (MEFs) revealed by an optimized new RNA bisulfite sequencing approach. Comparison of RNA methylation profile from wild-type and Dnmt2 -/- MEFs shows that only C38 in three tRNAs (tRNA-Asp, Gly and Val...
ORGANISM(S): Mus musculus 
2013-04-21 | E-GEOD-44359 | biostudies-arrayexpress
TranscriptomicsIdentification of direct targets and modified bases of RNA cytosine methyltransferases
We report development of a mechanism-based technique, Aza-IP, that utilizes the stable covalent linkage formed in vivo between an RNA methyltransferase (m5C-RMT) and 5-azacytidine (5-aza-C) incorporated within the target RNA to enable specific target enrichment, coupled with high-throughput sequenci...
ORGANISM(S): Homo sapiens 
2013-04-21 | E-GEOD-38957 | biostudies-arrayexpress
GenomicsRSC and ISW1 Chromatin Remodelers Display Functional and Chromatin-based Promoter Antagonism [MNase-Seq]
ISWI-family chromatin remodelers organize nucleosome arrays, while SWI/SNF-family remodelers (RSC) disorganize and eject nucleosomes, implying an antagonism that is largely unexplored in vivo. Here, we describe two independent genetic screens for rsc suppressors that yielded mutations in the promote...
ORGANISM(S): Saccharomyces cerevisiae 
2015-04-01 | E-GEOD-65593 | biostudies-arrayexpress
GenomicsRSC and ISW1 Chromatin Remodelers Display Functional and Chromatin-based Promoter Antagonism [ChIP-seq]
ISWI-family chromatin remodelers organize nucleosome arrays, while SWI/SNF-family remodelers (RSC) disorganize and eject nucleosomes, implying an antagonism that is largely unexplored in vivo. Here, we describe two independent genetic screens for rsc suppressors that yielded mutations in the promote...
ORGANISM(S): Saccharomyces cerevisiae 
2015-04-01 | E-GEOD-65592 | biostudies-arrayexpress
TranscriptomicsThe Dynamic Transcriptome of Schizosaccharomyces pombe Revealed by RNA/DNA Hybrid Mapping
We determined the strand-specific transcriptome of the fission yeast S. pombe under multiple growth conditions using a novel RNA/DNA hybridization mapping (HybMap) technique. HybMap uses an antibody against an RNA/DNA hybrid to detect RNA molecules hybridized to a high density DNA oligonucleotide ti...
ORGANISM(S): Schizosaccharomyces pombe 
2008-06-29 | E-GEOD-11619 | biostudies-arrayexpress
TranscriptomicsRNA Pol III transcriptomes and relationships to Pol II promoters, enhancer-binding factors and chromatin domains
RNA polymerase III transcribes many noncoding RNAs (e.g. tRNAs) important for translational capacity and other functions. Here, we localized RNA polymerase III, alternative TFIIIB complexes (BRF1/2) and TFIIIC in HeLa cells, determining the Pol III transcriptome, defining gene classes, and reveali...
ORGANISM(S): Homo sapiens 
2010-04-14 | E-GEOD-20609 | biostudies-arrayexpress
TranscriptomicsGenes for embryo development are packaged in blocks of multivalent chromatin in zebrafish sperm
In mature human sperm, genes of importance for embryo development (i.e. transcription factors) lack DNA methylation and bear nucleosomes with distinctive histone modifications, suggesting the specialized packaging of these developmental genes in the germline. Here, we explored the tractable zebrafis...
ORGANISM(S): Danio rerio 
2011-01-13 | E-GEOD-26609 | biostudies-arrayexpress
UnknownLong-term (180-Day) Outcomes in Critically Ill Patients With COVID-19 in the REMAP-CAP Randomized Clinical Trial.
Not available
S-EPMC9857594 | biostudies-literature
UnknownElective surgery cancellations due to the COVID-19 pandemic: global predictive modelling to inform surgical recovery plans.
Not available
S-EPMC7272903 | biostudies-literature
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