The hPDLSCs were cultured in the tenogenic medium treated with 0.1 ng/ml IL-1β or 10 ng/ml TNF-α for 7 days. Total proteins were isolated from the collected cell samples. Subsequently, the LFQ-based proteomic analysis was conducted for the extracted proteins in each group as established procedures...
The native microenvironment following osteochondral defect is not permissive for its endogenous regeneration. A variety of hydrogels have been developed to regulate cell behaviors; however, there is still a lack of hydrogels with multifunctional properties that can provide a supportive microenvironm...
Osteochondral defects (OCD) cannot be efficiently repaired due to the unique physical architecture and the pathological microenvironment including enhanced oxidative stress and inflammation. Conventional strategies, such as the control of implant microstructure or the introduction of growth factors,...
We performed stable isotope labeling of amino acids in cell culture (SILAC) and mass spectrometry (MS) analysis to identify potential acetylation sites on METTL3 in response to ectopic p300 expression.
