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ProteomicsSP3 protocol for proteomic plant sample preparation prior LC-MS/MS
Quantitative protein extraction from biological samples, as well as contaminants removal before LC-MS/MS, is fundamental for the successful bottom-up proteomic analysis. Four sample preparation methods, including the filter-aided sample preparation (FASP), two single-pot solid-phase-enhanced sample p...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-02-26 | PXD022688 | Pride
Protein cleanup Arabidopsis thaliana Bottom-up Mass spectrometry Sds removal Sp3 Fasp Carboxylated magnetic beads 
UnknownAnalytical methods of antibody surface coverage and orientation on bio-functionalized magnetic beads: application to immunocapture of TNF-α.
Not available
S-EPMC8367650 | biostudies-literature
ProteomicsArabidopsis thaliana peptide SP2 and ethyl acetate extraction clean up.
The success of bottom-up proteomic analysis frequently depends on the efficient removal of contaminants from protein or peptide samples before LC-MS/MS. For a peptide clean-up workflow, the single-pot solid-phase-enhanced peptide sample preparation on carboxylate-modified paramagnetic beads (termed ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-01-03 | PXD045031 | Pride
Arabidopsis thaliana Peptide clean-up Ethyl acetate extraction Sp2 Polyethylene glycol Sodium dodecyl sulfate Magnetic beads Lc-ms/ms 
UnknownSP3 Protocol for Proteomic Plant Sample Preparation Prior LC-MS/MS.
Not available
S-EPMC7988192 | biostudies-literature
ProteomicsAn optimized SP3 sample processing workflow for in-depth phos-phoproteomics and improved identification of multiply phosphory-lated peptides
Protein phosphorylation is a critical and ubiquitous post-translational modification (PTM) found across the kingdoms of life. Advances in high-throughput mass spectrometry have transformed our ability to interrogate the phosphoproteome. However, sample prepara-tion methodologies optimized for phosp...
ORGANISM(S): Homo sapiens (Human) 
2025-08-25 | PXD061013 | Pride
Phosphorylation  multiply phosphorylated  mass spectrometry  ptm  sp3  tio2  c18. 
UnknownBenchmarking of Two Peptide Clean-Up Protocols: SP2 and Ethyl Acetate Extraction for Sodium Dodecyl Sulfate or Polyethylene Glycol Removal from Plant Samples before LC-MS/MS.
Not available
S-EPMC10743447 | biostudies-literature
UnknownDNA aptamers against the Lup an 1 food allergen.
Not available
S-EPMC3328447 | biostudies-literature
GenomicsImpaired innate and adaptive immune responses to BNT162b2 SARSCoV-2 vaccination in systemic lupus erythematosus [CTD]
Impaired innate and adaptive immune responses to BNT162b2 SARSCoV-2 vaccination in systemic lupus erythematosus [CTD]
PRJNA1081858 | ENA
GenomicsImpaired innate and adaptive immune responses to BNT162b2 SARSCoV-2 vaccination in systemic lupus erythematosus [ACA]
Impaired innate and adaptive immune responses to BNT162b2 SARSCoV-2 vaccination in systemic lupus erythematosus [ACA]
PRJNA1081850 | ENA
ProteomicsR2-P2 rapid-robotic phosphoproteomics enables multidimensional cell signaling studies
Recent developments in mass spectrometry-based proteomics have enabled systems-level studies of cellular signaling, where >10,000 phosphosites can be routinely identified and quantified. Yet, current analyses are limited in throughput, reproducibility, and robustness, hampering experiments that invo...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-12-12 | PXD013453 | Pride
Phosphoproteomics / signaling / mapk / mass spectrometry / sp3 / dia/ r2-p2 
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