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ProteomicsQuantitative evaluation of FASP and MED FASP protocols
In this work we re-evaluate FASP and the related Multienzyme digestion (MED)-FASP method. We use different types of animal tissue and cultured cells, and test the performance of the method under various conditions. We analyze the protein-to-peptide conversion by assessing the yield of peptides, freq...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2016-04-28 | PXD003733 | Pride
Fasp  med fasp  missing cleavages  reproducibility 
ProteomicsGASP and FASP are complementary for LC-MS/MS proteomic analysis of drug-metabolising enzymes and transporters in pig liver
Sample preparation is a critical step in the proteomic workflow. Numerous different approaches are used, tailored to the type of sample, the aims of the experiment, analytical method, and to an extent, user preference. This has resulted in large variation in reported protein abundances. In this stud...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2018-11-12 | PXD011324 | Pride
Gasp Sample preparation Fasp Pig liver Lc-ms/ms 
ProteomicsEvaluation of FASP for mass spectrometry-based untargeted metabolomics analysis of urine samples
Filter-Aided Sample Preparation (FASP) is a well-established method in proteomics, yet its potential for the parallel recovery of metabolites remains largely unexplored. Herein, we evaluate the performance of FASP as a straightforward workflow for the simultaneous isolation of protein and correspond...
ORGANISM(S): Homo sapiens (Human) 
2026-06-01 | PXD079145 | Pride
Fasp  proteomics  metabolomics  multi-omics  bladder cancer  urine  sample preparation 
ProteomicsEvaluation of PAC and FASP performance
The objective of the experiment was to compare the performance of PAC and FASP.
ORGANISM(S): Pisum sativum (Garden pea) Glycine max Homo sapiens (Human) 
2024-06-16 | PXD044349 | Pride
Benchmarking Data-independent acquisition Protein aggregation capture Filter-aided sample preparation 
ProteomicsProtein Quantification and Identification from River Bank Grape, Vitis riparia: Comparing SDS-PAGE and FASP-GPF techniques for shotgun proteomic analysis.
The aim of this quantitative label-free shotgun proteomic experiment is to perform a comparative study of two different sample preparation methods which employ two different pre-fractionation techniques (SDS-PAGE and FASP-GPF) for use in shotgun proteomic analysis of Vitis riparia leaf material with...
ORGANISM(S): Vitis vinifera (Grape) Vitis riparia (Frost grape) (Vitis vulpina) 
2018-04-13 | PXD001399 | Pride
Sds-page Grapevine Fasp 
UnknownA modified FASP protocol for high-throughput preparation of protein samples for mass spectrometry.
Not available
S-EPMC5531558 | biostudies-literature
ProteomicsMStern Blot vs. FASP - Different sample types
Three different sample types (HeLa lysate, human CSF and human Urine) that were prepared with MStern Blot and FASP. For each sample type there are four replicates.
ORGANISM(S): Homo sapiens (Human) 
2015-07-30 | PXD002572 | Pride
Hela Csf Fasp Mstern blot Urine 
UnknownComparison of In-Solution, FASP, and S-Trap Based Digestion Methods for Bottom-Up Proteomic Studies.
Not available
S-EPMC9319029 | biostudies-literature
UnknownEnhanced FASP (eFASP) to increase proteome coverage and sample recovery for quantitative proteomic experiments.
Not available
S-EPMC3993969 | biostudies-literature
ProteomicsOn-bead vs FASP vs InGel sample preparation for LC-MS/MS
The aim of this project was to compare and optimize sample processing protocols up-stream of LC-MS/MS. We set out to create a protocol that is easier and less laborious to use with liquid handlers than FASP, but that generates comparable results as FASP or traditional in-gel digestion.
ORGANISM(S): Homo sapiens (Human) 
2018-07-19 | PXD005944 | Pride
On-bead digestion Gelfree Fasp Lc-ms/ms 
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