Enhanced FASP (eFASP) to increase proteome coverage and sample recovery for quantitative proteomic experiments.

ABSTRACT: The integrity of quantitative proteomic experiments depends on the reliability and the robustness of the protein extraction, solubilization, and digestion methods utilized. Combinations of detergents, chaotropes, and mechanical disruption can yield successful protein preparations; however, the methods subsequently required to eliminate these added contaminants, in addition to the salts, nucleic acids, and lipids already in the sample, can result in significant sample losses and incomplete contaminant removal. A recently introduced method for proteomic sample preparation, filter-aided sample preparation (FASP), cleverly circumvents many of the challenges associated with traditional protein purification methods but is associated with significant sample loss. Presented here is an enhanced FAS

SUBMITTER: Erde J 

PROVIDER: S-EPMC3993969 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature