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RNA was extracted from Hnf1a/Tcf1 (Hepatocyte Nuclear Factor 1 alpha) knockout and wild-type mouse liver fractions. Gene expression profiles were compared using Affymetrix GeneChip Mouse Genome 430 2.0 arrays [Mouse430_2].
ORGANISM(S): Mus musculus 
Pancreatic islets were isolated from Hnf1a (Hepatocyte nuclear factor 1 alpha) knockout and wild-type mice and cultured ex vivo for two days in RPMI medium with 10% FBS before RNA extraction. The transcription profiles were obtained using Affymetrix GeneChip Mouse Genome 430A 2.0 arrays [Mouse430A_2...
ORGANISM(S): Mus musculus 
Pancreatic islets were isolated from Hnf1a+/- (Hepatocyte nuclear factor 1 alpha) and wild-type mice and cultured ex vivo for two days in RPMI medium with 10% FBS before RNA extraction. The transcription profiles were obtained using Affymetrix GeneChip Mouse Genome 430 2.0 arrays [Mouse430_2].
ORGANISM(S): Mus musculus 
Chromatin from Hnf1a-/- and wild-type mouse hepatocytes was immunoprecipitated with antibodies anti-H3K4me2 (Upstate 07-030). Amplified and labelled ChIP DNA and input DNA were hybridyzed to Mouse PromoterChip 5A.0 arrays.
ORGANISM(S): Mus musculus 
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