Cardoso Carlos C

OmicsDI

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Unknown (124)
Transcriptomics (6)
Metabolomics (1)

Organisms

Mus musculus (5)
Homo sapiens (1)

Repository

biostudies-arrayexpress (6)
MetaboLights (1)

Technology Type

Mass spectrometry (1)

Instrument Platform

Single-Cell Extraction: For enzymatic digestion, pronase (Septomyces argeus, 1 mg/mL) was applied for 25 minutes at room temperature for P0 to P8 datasets. Cells were dissociated and triturated into a single-cell suspension in ACSF supplemented with 1% fetal calf serum (FCS) and DNase (1 µL/10 mL). Trituration was performed using three glass Pasteur pipettes of decreasing diameters. For P30 datasets, enzymatic digestion and cell dissociation were carried out using the Worthington Papain Dissociation System, following the manufacturer’s instructions. Single-Nuclei Extraction: For nuclei isolation, the Hibernate™-E Medium was removed, and 500 µL of chilled 0.1X NP40 Lysis Buffer was added. Tissue was homogenized using a Pellet Pestle (15 strokes) and incubated on ice for 5 minutes. The suspension was pipette-mixed 10 times using a wide-bore pipette tip and incubated for an additional 10 minutes on ice. Following lysis, 500 µL of chilled wash buffer was added, and the mixture was pipette-mixed 5 times using a regular-bore pipette tip. The suspension was filtered through a 30 µm cell strainer into a 50 mL tube to remove debris. The filtered suspension was transferred to a 1.5 mL tube and centrifuged at 950 × g for 10 minutes at 4°C. The supernatant was carefully removed, and the nuclei pellet was retained for downstream analysis. (2)
Illumina HiSeq 4000 (2)
NextSeq 500 (2)
Chromium Next GEM Chip G (1)
10X v3 (1)
Liquid Chromatography MS - positive - reverse phase (1)

Publication Date

2021 (3)
2026 (1)
2025 (1)
2022 (1)
2014 (1)

CHEBI ID

CHEBI:111173 (1)
CHEBI:133530 (1)
CHEBI:141538 (1)
CHEBI:15843 (1)
CHEBI:169465 (1)
CHEBI:172722 (1)
CHEBI:180545 (1)
CHEBI:180546 (1)
CHEBI:180548 (1)
CHEBI:180549 (1)
CHEBI:28177 (1)
CHEBI:31804 (1)
CHEBI:32095 (1)
CHEBI:34162 (1)
CHEBI:45996 (1)
CHEBI:53460 (1)
CHEBI:61205 (1)
CHEBI:69188 (1)
CHEBI:73731 (1)
CHEBI:73944 (1)
CHEBI:75216 (1)
CHEBI:75555 (1)
CHEBI:79169 (1)
CHEBI:82470 (1)
CHEBI:82984 (1)
CHEBI:84883 (1)
CHEBI:85208 (1)
CHEBI:9242 (1)

Metabolite Name

(11Z,14Z)-Eicosadienoic acid (1)
1-Meadoyl-glycero-3-phosphate (1)
1-O-Octadecylglycerol-3-phosphatidylcholine (1)
12-Hydroxystearic acid (1)
15-Hydroxypentadecanoic acid (1)
16(R)-HETE (1)
2,3-Dihydroxypropyl stearate (1)
2,3-Dinor-6-oxoprostaglandin F1alpha (1)
3-(Tert-butyl)-N-(2,3-dihydro-1-benzofuran-5-ylmethyl)-1-methyl-1H-pyrazole-5-carboxamide (1)
4-Hydroxy-16,18-tritriacontanedione (1)
4-Undecylbenzenesulfonic acid (1)
Alpha-Pyrrolidinopropiophenone (1)
Arachidonic acid (1)
Boc-D-Asparagine (1)
Dihomo-alpha-linolenic acid (1)
Docosatetraenoic acid (1)
Ethyl eicosapentaenoic acid (1)
Glycidyl Stearate (1)
Hexyl furoate (1)
Keratan sulfate I (1)
Linoleamide (1)
Mebutamate (1)
Methyl stearate (1)
Retinol acetate (1)
Ribothymidine (1)
Spiroxamine (1)
Theophylline (1)
Tyramine-O-sulfate (1)

Study type

Transcription profiling by array (2)
RNA-seq of coding RNA from single cells (2)
Animal - Single-cell sequencing (1)
Animal - High-throughput sequencing (1)

Release Date

2021 (27)
2022 (18)
2024 (18)
2025 (16)
2023 (11)
2020 (9)
2018 (5)
2017 (5)
2016 (5)
2019 (4)
2012 (3)
2026 (2)
2014 (2)
2015 (2)
2013 (1)
2010 (1)
2009 (1)

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Case of acute hepatitis E with concomitant signs of autoimmunity.

Not available

S-EPMC3612576 | biostudies-literature

Sarcopenic metabolomic profile reflected a sarcopenic phenotype associated with amino acid and essential fatty acid changes

INTRODUCTION: Although sarcopenia greatly affects health and quality of life in older people, its pathophysiological causes are not fully elucidated. To face this challenge, omics technologies can be used. The metabolome gives a vision of the interaction between the genome and t...

2021-09-29 | MTBLS2679 | MetaboLights

The C-terminal extension of VgrG4 from Klebsiella pneumoniae remodels host cell microfilaments.

Not available

S-EPMC9542434 | biostudies-literature

5-Methyl-2,4-bis-(methyl-sulfan-yl)tricyclo-[6.2.1.0]undeca-4,9-diene-3,6-dione.

Not available

S-EPMC2979396 | biostudies-literature

Uncovering the Molecular Logic of Cortical Wiring between Neuronal subtypes Across Development Through Ligand–Receptor Inference

The cerebral cortex comprises diverse excitatory and inhibitory neuron subtypes, each with distinct laminar positions and connectivity patterns. Yet, the molecular logic underlying their precise wiring remains poorly understood. To identify ligand–receptor (LR) interactions involved in cortical circ...

ORGANISM(S): Mus musculus

2026-01-30 | E-MTAB-16260 | biostudies-arrayexpress

Crystallization and preliminary X-ray diffraction analysis of endoglucanase III from Trichoderma harzianum.

Not available

S-EPMC3310537 | biostudies-literature

ClearColi as a platform for untagged pneumococcal surface protein A production: cultivation strategy, bioreactor culture, and purification.

Not available

S-EPMC8755982 | biostudies-literature

The assessment of lifestyle changes during the COVID-19 pandemic using a multidimensional scale.

Not available

S-EPMC7456305 | biostudies-literature

The assessment of lifestyle changes during the COVID-19 pandemic using a multidimensional scale.