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ProteomicsCBB staining showed beneficial effects on proteomic analysis employing PAGE separation followed with whole-gel slicing, in-gel digestion and quantitative LC-MS/MS
Proteomic analysis by combining PAGE separation, gel slicing and slice-by-slice LC-MS/MS has been frequently reported in recent years. Since the MS analysis would provide identities and quantities of the proteins along the whole lane, visualization by dye staining could be skipped to save time and ...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-02-16 | PXD011689 | Pride
Sds-page In-gel digestion Proteomic analysis Cbb r-250 staining Whole gel slicing Quantitative lc-ms/ms Nondenaturing page 
ProteomicsImmunoprecipitation followed by mass spectrometry (IP-MS)
Immunoprecipitation of ITGB1 and YWHAE was done to understand its interactive partners in K562/S cells. Mass spectrometry was performed to identify interactive molecules. Non-specific proteins (contaminants) such as keratins were excluded from the final analysis based on the results of the negative ...
ORGANISM(S): Homo sapiens (Human) 
2025-10-30 | PXD056828 | Pride
Immunoprecipitation Mass spectrometry Protein-protein interaction Lc-ms/ms 
ProteomicsThe effect of aqueous extract Gynura procumbens on fertility of diabetes induced male rats using proteomic approach
Medicinal plants or herbs have been long used in traditional practice and considered as an alternative treatment for diseases. Gynura procumbens is one of the herbs that showed high potential for treating various diseases such as diabetes mellitus, cancer and fertility. Previous study demonstrated G...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-05-20 | PXD011373 | Pride
Fertility Gynura procumbens Proteomic Diabetes mellitus 
ProteomicsAnalysis of low molecular weight protein profile in whole saliva with special reference to ovulation
Whole saliva is contains major, minor and gingival crevicular fluid/secretions and it plays vital role in maintaining the oral health, three pair of major salivary glands consist of parotid, submandibular and sub-lingual glands. Saliva is a good biological fluid to explore health and disease stat...
ORGANISM(S): Homo sapiens (Human) 
2016-12-22 | PXD000921 | Pride
Saliva Lc-msms Ovulation Protein Cystatin 
ProteomicsH2S regulation the metabolism of cucumber in response to salt-stress through transcriptome and proteome analysis
In a previous study, we found that H2S alleviates salinity stress in cucumber by maintaining the Na+/K+ balance and by regulating H2S metabolism and the oxidative stress response. However, little is known about the molecular mechanisms behind H2S-regulated salt-stress tolerance in cucumber. Here, an...
ORGANISM(S): Cucumis sativus 
2021-09-09 | PXD020642 | Pride
Transcriptomic Proteomic  hydrogen sulfide Cucumis sativus l. Salt stress 
ProteomicsThe salivary proteome profile in women throughout the menstrual cycle characterized by HR-LC-MS/MS
Generally saliva consists of mixture of proteins electrolytes and small organic compounds. This whole saliva stands for secretions from major (submandibular, sublingual and parotid) and minor salivary glands, along with crevicular fluid, bacteria and cellular debris. The secretion of saliva is regul...
ORGANISM(S): Homo sapiens (Human) 
2021-11-25 | PXD004511 | Pride
Protein Saiva Lc-ms/ms Ovulation 
ProteomicsAnalysis of LDL-associated proteins using the method of digitized native protein mapping
The method of digitized native protein mapping, combining nondenaturing micro 2DE, grid gel-cutting and quantitative LC-MS/MS (in data-independent acquisition mode, or MSE), was improved by using a new MS/MS mode, ion mobility separation enhanced-MSE (HDMSE) and applied to analyze the area of human ...
ORGANISM(S): Homo sapiens (Human) 
2016-05-17 | PXD003720 | Pride
Grid gel-cutting Quantitative lc-hdmse Human plasma low-density lipoprotein Protein-protein interactions Nondenaturing micro 2de Digitized native protein mapping 
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