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MetabolomicsDissection of two routes to naïve pluripotency using different kinase inhibitors
Embryonic stem cells (ESCs) can be maintained in the naïve state through inhibition of Mek1/2 and Gsk3 (2i). A relevant effect of 2i is the inhibition of Cdk8/19, which are negative regulators of the Mediator complex, responsible for the activity of enhancers. Inhibition of Cdk8/19 (Cdk8/19i) stimul...
2021-02-10 | MTBLS301 | MetaboLights
TranscriptomicsRole of CDK8 in interferon-gamma-induced gene expression
Gene regulation by cytokine-activated STAT transcription factors requires serine phosphorylation within the transactivation domain (TAD). STAT1 and STAT3 TAD phosphorylation was reported to occur upon promoter binding by an unknown kinase. Here we show that the Mediator CDK8 module phosphorylates S7...
ORGANISM(S): Mus musculus 
2013-02-28 | E-GEOD-40728 | biostudies-arrayexpress
TranscriptomicsIdentification of a potent and selective chemical probe for exploring the role of Mediator complex-associated protein kinases CDK8 and CDK19 in human disease [Colo205]
There is an unmet need for chemical tools to explore the role of the Mediator complex in human pathologies ranging from cancer to cardiovascular disease. Here we determine that CCT251545 a WNT-pathway inhibitor discovered by cell-based screening is a potent and selective chemical probe for the Medi...
ORGANISM(S): Homo sapiens 
2015-10-26 | E-GEOD-67845 | biostudies-arrayexpress
TranscriptomicsIdentification of a potent and selective chemical probe for exploring the role of Mediator complex-associated protein kinases CDK8 and CDK19 in human disease [LS-174-T]
There is an unmet need for chemical tools to explore the role of the Mediator complex in human pathologies ranging from cancer to cardiovascular disease. Here we determine that CCT251545 a WNT-pathway inhibitor discovered by cell-based screening is a potent and selective chemical probe for the Medi...
ORGANISM(S): Homo sapiens 
2015-10-26 | E-GEOD-67846 | biostudies-arrayexpress
TranscriptomicsIdentification of a potent and selective chemical probe for exploring the role of Mediator complex-associated protein kinases CDK8 and CDK19 in human disease [beta-catenin organoids]
There is an unmet need for chemical tools to explore the role of the Mediator complex in human pathologies ranging from cancer to cardiovascular disease. Here we determine that CCT251545 a WNT-pathway inhibitor discovered by cell-based screening is a potent and selective chemical probe for the Medi...
ORGANISM(S): Mus musculus 
2015-10-26 | E-GEOD-67847 | biostudies-arrayexpress
ProteomicsThe Cdk8 kinase module regulates Mediator-RNA polymerase II interaction
We use biochemically reconstituted transcription initiation components including the Mediator core and Cdk8 kinase modules (CKM) to investigate the function of the CKM in regulating the Mediator-RNA polymerase II interaction in a highly purified system. We use cross-linking coupled to mass spectrome...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-04-28 | PXD023361 | Pride
Transcription Mediator Cdk8 Rna polymerase ii 
TranscriptomicsCDK8 knockdown in HT-29 cells and CDK8 & MED12 knockdown in R1 cells
This SuperSeries is composed of the following subset Series: GSE30815: CDK8 knockdown in HT-29 human colon cancer cells GSE30816: CDK8 and MED12 knockdown in R1 mouse ES cells Refer to individual Series
ORGANISM(S): Mus musculus 
2012-03-05 | E-GEOD-30817 | biostudies-arrayexpress
ProteomicsProteomic profiling of CDK8 Inhibition
Acute myeloid leukemia (AML) with leukemic stem cell (LSC) characteristics represents a therapeutic challenge due to inherent resistance to differentiation. CDK8, a kinase component of the Mediator complex, has emerged as a regulator of oncogenic transcription, yet its role in LSC maintenance and po...
ORGANISM(S): Homo sapiens (Human) 
2026-09-14 | PXD074404 | Pride
Phosphopeptides Chromatin immunoprecipitation Leukemic stem cell Cdk8 kinase Interactome 
TranscriptomicsExpression profile in wild type, fkh2delta and fkh2-S2A mutants
Mitotic entry is accompanyed by the expression of a cluster of so called mitotic genes, whose activation is critical for mitosis in human and yeast cells. We found a link between the transcription machinery and cell cycle control network at mitosis in fission yeast, involving the Cdk8 kinase depende...
ORGANISM(S): Schizosaccharomyces pombe 
2012-07-12 | E-GEOD-31642 | biostudies-arrayexpress
GenomicsA precisely positioned MED12 activation helix stimulates CDK8 kinase activity
A precisely positioned MED12 activation helix stimulates CDK8 kinase activity
PRJNA558963 | ENA
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