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ProteomicsPhosTip for Enrichment of Phosphopeptides from Urinary Extracellular Vesicles
Extracellular vesicles (EVs) are attracting increasing interest with their intrigue role in intercellular communications. Protein phosphorylation in EVs is of great importance for understanding intercellular signaling processes. However, the study of EV phosphoproteomics is impeded by their relative...
ORGANISM(S): Homo sapiens (Human) 
2022-02-15 | PXD020861 | Pride
Phosphopeptides， extracellular vesicles，biomarker 
ProteomicsModulating the selectivity of affinity absorbents to multi-phosphopeptides by a novel competitive substitution strategy
Although many affinity adsorbents have been developed for phosphopeptides enrichment, high-specifically capturing the multi-phosphopeptides is still a big challenge. Here, we investigated the mechanism of phosphate ions coordination and substitution on affinity adsorbents surfaces and modulated the ...
ORGANISM(S): Homo sapiens (Human) 
2016-08-03 | PXD004252 | Pride
Mass spectrometry Multi-phosphopeptide enrichment Pre-coordination Phosphoproteomics Competitive substitution 
ProteomicsModulating the selectivity of affinity absorbents to multi-phosphopeptides by a novel competitive substitution strategy
Although many affinity adsorbents have been developed for phosphopeptides enrichment, high-specifically capturing the multi-phosphopeptides is still a big challenge. Here, we investigated the mechanism of phosphate ions coordination and substitution on affinity adsorbents surfaces and modulated the ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080735 | MassIVE
ProteomicsAnion-Exchange Chromatography of Tryptic and Phosphopeptides: WAX vs. SAX and AEX vs. ERLIC
At a pH > 5, phosphopeptides have two negative charges per residue and are well-retained in anion-exchange chromatography. However, the peptides with one or two phosphate groups are not separated from the peptides with multiple Asp or Glu residues, which interfere with the identification of phosph...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080774 | MassIVE
UnknownSynthesis and bioactivity of pyrrole-conjugated phosphopeptides.
Not available
S-EPMC8822458 | biostudies-literature
ProteomicsATP-Coated Dual-functionalized Titanium (IV) IMAC Material for Simultaneous Enrichment and Separation of Glycopeptides and Phosphopeptides
Protein glycosylation and phosphorylation are two of the most common post-translational modifications (PTMs), which plays an important role in many biological processes. However, low abundance and poor ionization efficiency of phosphopeptides and glycopeptides make direct MS analysis challenging. Pr...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-07-20 | PXD029775 | Pride
Phosphoproteomics Lc-ms/ms Glycoproteomics 
UnknownDeveloping Workflow for Simultaneous Analyses of Phosphopeptides and Glycopeptides.
Not available
S-EPMC6624649 | biostudies-literature
UnknownModification of Luffa Sponge for Enrichment of Phosphopeptides.
Not available
S-EPMC6982136 | biostudies-literature
ProteomicsPKA-regulated Phosphopeptides research in Pheromone gland of Helicoverpa armigera
PKA-regulated Phosphopeptides research used Itraq method in Pheromone gland of Helicoverpa armigera
ORGANISM(S): Helicoverpa armigera (Cotton bollworm) 
2017-10-10 | PXD006034 | Pride
Proteomics Helicoverpa armigera Pheromone gland Phosphopeptides Itraq 
ProteomicsFractionation of phosphopeptides using reversed phase microcolumn prior to LC-MS analysis
A protocol for an improved phosphopeptide identification in tryptically digested complex peptide samples is described. The common TiO2 based phoshopeptide enrichment is coupled to a simple peptide fractionation protocol with following LC-MS analysis of the obtained fractions and proteomic identifica...
ORGANISM(S): Homo sapiens (Human) 
2020-08-31 | PXD018663 | Pride
Phosphopeptide fractionation H1299 cell line Phosphorylation 
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