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MetabolomicsCryogenic OrbiSIMS Localizes Semi-Volatile Molecules in Biological Tissues
OrbiSIMS is a recently developed instrument for label-free imaging of chemicals with micron spatial resolution and high mass resolution. We report a cryogenic workflow for OrbiSIMS (Cryo-OrbiSIMS) that improves chemical detection of lipids and other biomolecules in tissues. Cryo-OrbiSIMS boosts ioni...
2023-03-15 | MTBLS1873 | MetaboLights
MetabolomicsSuboxic DOM is bioavailable to surface prokaryotes in a simulated overturn of an oxygen minimum zone, Devil’s Hole, Bermuda
Oxygen minimum zones (OMZs) are expanding due to increased sea surface temperatures, subsequent increased oxygen demand through respiration, reduced oxygen solubility, and thermal stratification driven in part by anthropogenic climate change. Devil's Hole, Bermuda is a model ecosystem to study OMZ m...
2024-02-27 | MTBLS8122 | MetaboLights
MetabolomicsCharacterization of Phytoplankton-Excreted Metabolites Mediating Carbon Flux through the Surface Ocean

The marine labile dissolved organic carbon (DOC) pool is a dynamic reservoir of thousands of molecules. After excretion or release by phytoplankton and other microbes, metabolites are rapidly consumed, resulting in very low standing concentrations (picomolar to low nanomolar). To date, marine phy...

2026-03-17 | MTBLS12895 | MetaboLights
MetabolomicsMetabolites from surface seawater collected in Narragansett Bay
In aquatic environments, the production and consumption of organic compounds is directly tied to the metabolic potential of the in situ microbial community. The community’s metabolic potential can be assessed using metatranscriptomics, which is a measure of gene expression in the environment. More r...
2016-01-14 | MTBLS293 | MetaboLights
MetabolomicsMass Spectrometry Imaging of Lipids in a Gut Epithelial Cell Model

Scope: The Caco2/HT29-MTX co-culture system is widely used as a cell model of the intestinal epithelium. Although the gut epithelium plays an important role in the uptake of free fatty acids and the resynthesis of triglycerides, the lipid distribution profile of the two-dimensional co-culture sys...

2025-06-14 | MTBLS12599 | MetaboLights
MetabolomicsStable Isotope-Assisted Metabolomics for Deciphering Xenobiotic Metabolism in Mammalian Cell Culture
Xenobiotics are ubiquitous in the environment and modified abundantly in the human body by phase I and II metabolism. Liquid chromatography coupled to high resolution mass spectrometry is a powerful tool to investigate these biotransformation products. Here, we present a workflow based on stable iso...
2020-04-14 | MTBLS1358 | MetaboLights
TranscriptomicsHIT: a new versatile protein array platform for multi-analyte phenotyping of surface molecules
We have developed a multi-analyte fluid-phase protein array technology termed high-throughput immunophenotyping using transcription (HIT). This method employs a panel of monoclonal antibodies, each tagged with a unique oligonucleotide sequence that serves as a molecular barcode. After staining a s...
ORGANISM(S): Homo sapiens 
2010-05-26 | E-GEOD-10665 | biostudies-arrayexpress
ProteomicsComprehensive identification of full-length mRNA isoforms reveals massive diversity of neural cell-surface molecules and illuminates their function in development and disease
Genes encoding cell-surface proteins are crucial for nervous system development and are implicated in neurological disorders. These genes produce alternative mRNA isoforms which remain poorly characterized, impeding our understanding of how disease-associated mutations cause pathology. Here we intro...
ORGANISM(S): Mus musculus (Mouse) 
2020-08-11 | PXD017290 | Pride
Cell-surface molecules Neural development Pacbio sequencing Isoforms 
MetabolomicsAlterations in the metabolic footprint induced by Mayaro virus infection in cell culture
This study focuses metabolic footprinting of Vero cells infected by Mayaro virus. Nuclear magnetic resonance combined with multivariate analytical methods and tools of pattern recognition shows that metabolic changes can be attributed to the effect of Mayaro virus infection. Vero cells infected by M...
2021-10-28 | MTBLS639 | MetaboLights
MetabolomicsMycobacterium tuberculosis-derived linoleic acid increases regulatory T cell function to promote bacterial survival within macrophages

Regulatory T cells (Tregs) expand during Mycobacterium tuberculosis (Mtb) infection and suppress T cell mediated control. Whether Mtb actively contributes to this process is unclear. Here, using a genome-wide mutant library, we show that expression of Mtb Rv1272c, an ATP-binding cassette transpor...

2025-09-28 | MTBLS12875 | MetaboLights
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