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ProteomicsEngineering cell-free systems by chemoproteomic-assisted phenotypic screening.
Phenotypic screening is a valuable tool to both understand and engineer complex biological systems. We demonstrate the functionality of this approach in the development of cell-free protein synthesis (CFPS) technology. Phenotypic screening identified numerous compounds that enhanced protein produc...
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) 
2024-07-22 | PXD047986 | JPOST Repository
ProteomicsSer/Leu-swapped cell-free translation system constructed with natural/in vitro transcribed-hybrid tRNA set
Analysis of the peptides and proteins synthesized in a cell-free translation system containing either the natural tRNA extract, the hybrid-SL tRNA set, or the hybrid-SL tRNA set minus one chimeric tRNA using LC-MS.
ORGANISM(S): Cellular Organisms 
2024-04-08 | PXD050604 | JPOST Repository
MetabolomicsProteomic analysis validates previous findings on wounding-responsive plant hormone signaling and primary metabolism contributing to the biosynthesis of secondary metabolites based on metabolomic analysis in harvested broccoli (Brassica oleracea L. var. italica)
The plant wound-response is a complex process that generates physiological modifications for protecting the wounded tissue. In this study, tandem mass tag (TMT)-based quantitative proteomic analysis was performed to clarify the comprehensive molecular mechanism for the wound-response of broccoli subj...
2021-05-19 | MTBLS2317 | MetaboLights
ProteomicsAssembly and cell-free expression of a partial genome for the synthetic cell
We designed and assembled a multicistronic DNA plasmid containing multiple genes that are relevant for a synthetic cell. This partial minimal synthetic genome was expressed in the recombinant PURE system. Cell-free biosynthesis of all encoded proteins was validated by liquid chromatography-mass spec...
ORGANISM(S): Anaplasma marginale Aequorea victoria Bacillus phage phi29 Escherichia coli 
2026-10-28 | PXD069407 | Pride
Partial genome Cell-free biosynthesis Label-free quantification Synthetic cell Lc-ms/ms 
UnknownA Cell-Free Platform Based on Nisin Biosynthesis for Discovering Novel Lanthipeptides and Guiding their Overproduction In Vivo.
Not available
S-EPMC7507342 | biostudies-literature
ProteomicsIn vitro synthesis of 32 translation-factor proteins from a single template reveals impaired ribosomal processivity
The Protein synthesis Using Recombinant Elements (PURE) system enables transcription and translation of a DNA template from purified components. Therefore, the PURE system-catalyzed generation of RNAs and proteins constituting the PURE system itself represents a major challenge toward a self-replica...
ORGANISM(S): Escherichia Coli 
2021-09-06 | PXD020877 | panorama
MetabolomicsDeep longitudinal multi-omics analysis of Bordetella pertussis cultivated in bioreactors highlights medium starvations and transitory metabolisms, associated to vaccine antigen biosynthesis variations and global virulence regulation

Bordetella pertussis is the bacterial causative agent of whooping cough, a serious respiratory illness. An extensive knowledge on its virulence regulation and metabolism is a key factor to ensure pertussis vaccine manufacturing process robustness. The aim of this study was to refine our ...

2023-02-21 | MTBLS5762 | MetaboLights
UnknownCell-Free Phospholipid Biosynthesis by Gene-Encoded Enzymes Reconstituted in Liposomes.
Not available
S-EPMC5053487 | biostudies-literature
TranscriptomicsTranscription profiling of wild-type and Rb-/- prostate epithelial cell lines under untreated and serum-free conditions.
Comparison of control wild-type and Rb-/- prostate epithelial cell lines under untreated and serum-free conditions.
ORGANISM(S): Mus musculus 
2007-12-13 | E-GEOD-934 | biostudies-arrayexpress
TranscriptomicsThe Zinc Finger Transcription Factor SlZFP2 Negatively Regulates Abscisic Acid Biosynthesis and Fruit Ripening in Tomato
Abscisic acid (ABA) regulates plant development and adaptation to environmental conditions. The ABA biosynthesis pathway in plants has been thoroughly elucidated; however, very few transcription factors directly regulating the expression of ABA biosynthetic genes have been identified. Here we show t...
ORGANISM(S): Solanum pimpinellifolium 
2015-11-23 | E-GEOD-63838 | biostudies-arrayexpress
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