HEK293T cells were co-transfected with StrepII-FLAG-tagged DCAF12 and HA-tagged CUL4A, grown for 48 h, harvested, and lysed as described above. In the first step, Strep-TactinXT Superflow resin (IBA Lifesciences, Gottingen, Germany) was used to purify proteins associated with StrepII-FLAG-tagged sub...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)