Sort   by:  
 Page size 
GenomicsOrganoid cultures of early-onset colorectal cancers reveal distinct and rare genetic profiles
Sporadic early-onset colorectal cancer (EOCRC) has bad prognosis, yet is poorly represented by cell line models. We examine the key mutational and transcriptomic alterations in an organoid biobank enriched in EOCRCs. We established paired cancer (n=32) and normal organoids (n=18) from 20 patients en...
EGAS00001004063 | EGA
Genomicsena-DATASET-THE UNIVERSITY OF HONG KONG DEPARTMENT OF PATHOLOGY AND PFIZER INC.-18-12-2019-11:12:21:554-1297 - samples
Four micrograms of total RNA was used for cDNA library construction using the KAPA Stranded mRNA-Seq Kit (KR0960-v3.15), following manufacturer's protocol. The adaptor-ligated libraries were enriched by 6 cycles of polymerase chain reaction (PCR). Libraries were sequenced using the Novaseq 6000 with...
EGAD00001005753 | EGA
Genomicsena-DATASET-THE UNIVERSITY OF HONG KONG DEPARTMENT OF PATHOLOGY AND PFIZER INC.-20-12-2019-08:50:56:915-1359 - samples
Five hundred nanograms of genomic DNA was fragmented by Covaris S2, the fragmented DNAs were performed end-repair, A-tailing at the 3 prime end, adaptors ligation with an IDT dual-indexed UMI adaptor system at the terminal ends. The adapter ligated library with size range 300-750bp were selected by...
EGAD00001005759 | EGA
Genomicsena-DATASET-THE UNIVERSITY OF HONG KONG DEPARTMENT OF PATHOLOGY AND PFIZER INC.-18-12-2019-11:18:36:686-1298 - samples
Five hundred fifty nanograms of genomic DNA were input for library preparation after fragmentation by Covaris S2, following the KAPA Hyper Prep Kit (KR0961-V1.14) protocols, with selection for a library size range of 250-450 bp. Three hundred nanograms per library DNA each from 12 samples were norma...
EGAD00001005754 | EGA
GenomicsThe University of Hong Kong Colon Cancer Organoids Genomics Study Data Access Committee
Data Access Committee EGAC00001001405
EGAC00001001405 | EGA
Sort   by:  
 Page size