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We developed a tip-based sequential IMAC based on different kinds of metal ion for increasing the coverage of phosphoproteome. This approach not only can be used for singly and multiply phosphopeptides separation but also for acidic, basophilic and Pro-directed phosphopeptides separation.
ORGANISM(S): Homo Sapiens (human) 
Untargeted data independent acquisition proteomics analysis using Orbitrap mass spectrometers and DIA-Umpire
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-04-01 | MSV000080875 | MassIVE
Sequential Phosphoproteomic Enrichment by Complementary MD-IMAC, Cancer tissues
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005323 | PeptideAtlas
Sequential Phosphoproteomic Enrichment by Complementary MD-IMAC, Raji cell line
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005320 | PeptideAtlas
Plant phosphoproteomics reveals a global view of phosphorylation-mediated signaling in plants, but it remains demanding a high-throughput method with detection sensitivity and quantification accuracy. In general, the indispensable protein precipitation step requiring for interferences removal limite...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
Mining Missing Membrane Proteins, Hp-RP StageTips, CL83
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005364 | PeptideAtlas
Mining Missing Membrane Proteins, Hp-RP StageTips, PC9
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005379 | PeptideAtlas
Mining Missing Membrane Proteins, Hp-RP StageTips, A549
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005358 | PeptideAtlas
Mining Missing Membrane Proteins, Hp-RP StageTips, CL97
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005449 | PeptideAtlas
Mining Missing Membrane Proteins, Hp-RP StageTips, CL100
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005443 | PeptideAtlas
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