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ProteomicsTyrosine phosphorylation site-specific interactome of adaptor protein Shc1 explored by synthetic protein domains
As a critical adaptor protein, Shc1 contains SH2 and PTB domains for scaffolding tyrosine phosphorylation-dependent protein complexes, therefore regulating growth factor receptor signaling networks. The three tyrosine phosphorylation sites (pY) on the CH1 linker region of SHC1 provide additional doc...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2024-08-15 | PXD049113 | Pride
Tyrosine phosphorylation Signal transduction Shc1 Mass spectrometry Ch1 region 
ProteomicsGlycosylation detection of carcinoembryonic antigen in human plasma
We develop a method with high sensitivity and reproducibility to comprehensively illustrated the glycosylation of plasma CEA based on trifunctional probe and multi-enzymatic approach. Site-specific glycoforms of plasma CEA differed in patients with different cancers and the progression of CRC.
ORGANISM(S): Homo Sapiens 
2023-10-31 | PXD046518 |
Proteomics[HPP-2.0] Integrated and high-throughput approach for sensitive analysis of tyrosine phosphoproteome
In this study, we developed a 96-well microplate-based approach to integrate all the sample preparation steps starting from cell culture to MS-compatible pTyr peptide enrichment in three consecutive 96-well microplates. By assembling engineered SH2 domain onto microplate, non-specific adsorption of ...
ORGANISM(S): Homo Sapiens 
2024-02-03 | PXD049140 |
ProteomicsDeKinomics pulse-chases kinase functions in living cells
Cellular context is crucial for understanding the complex and dynamic kinase functions in health and disease. Systematic dissection of kinase-mediated cellular processes requires rapid and precise stimulation (“pulse”) of a kinase of interest, as well as global and in-depth characterization (“chase”...
ORGANISM(S): Homo Sapiens 
2023-10-07 | PXD039316 |
Proteomics[HPP-2.0] Motif-dependent immune co-receptor interactome profiling by photoaffinity chemical proteomics
Phosphotyrosine Motif-dependent synthetic CD28 interactome
ORGANISM(S): Homo Sapiens 
2024-02-06 | PXD049270 |
ProteomicsMultimodal single-cell resolved spatial proteomics reveal pancreatic tumor heterogeneity
In this study, we offer a competitive solution for uncovering the comprehensive heterogeneity of tissue context.
ORGANISM(S): Mus Musculus 
2024-10-23 | PXD038882 |
ProteomicsSpatiotemporal Profiling of Cytosolic Signaling Complexes in Living Cells by Highly Selective Proximity Proteomics
APEX2-mediated proximity labeling pioneers in situ capture of spatiotemporal protein complexes in living cells but is limited by its high background. Here, we developed an optimized probe BP5 with much lower labeling noise. Combining the new proximity labeling with label-free quantitative proteomics...
ORGANISM(S): Homo sapiens (Human) 
2020-11-18 | PXD020709 | Pride
Protein complex Proximity labeling Spatiotemporal Proteomics 
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