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Protein phosphorylation and N-glycosylation are key post-translational modifications (PTMs) in plants that regulate critical signaling processes. However, analyzing both PTMs simultaneously remains challenging due to low abundance and divergent enrichment requirements. Here, we present a single-tip ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-05-14 | PXD061247 | Pride
In present work, we established a simple and robust protocol for universal bacterial phosphoproteomic analysis underlying the rationale of methanol-based LLE protein extraction and cleanup. Using the SDS lysis as the conventional protocol control, we carefully evaluated the efficiency of protein ext...
ORGANISM(S): Escherichia Coli Cellular Organisms 
Mass spectrometry (MS)-based single-cell proteomics (SCP) provides us the opportunity to unbiasedly explore biological variability within cells without the limitation of antibody availability. This field is rapidly developed with the main focuses on instrument advancement, sample preparation refinem...
ORGANISM(S): Homo Sapiens (human) 
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