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TranscriptomicsHT-SELEX of recombinant C2H2 zinc finger domains of Sall4
To define the sequence preference of SALL4 C2H2 zinc finger domains, we performed SELEX coupled with high-throughput sequencing (HT-SELEX) using the purified SALL4 ZFC1, ZFC2 and ZFC4 domains combined with no protein control experiment.
ORGANISM(S): artificial sequences 
2021-01-05 | E-MTAB-9236 | biostudies-arrayexpress
TranscriptomicsChIP-Seq of ES-E14 mouse embryonic stem cells investigating changes in Sall4
Sall4 is a stem cell factor which is important for embryogenesis. We have genetically modified Sall4 in mouse embryonic stem cells to access the preference of Sall4 binding site. There are three different genetic modifications for the ES cells in the form of Sall4 Knockout (KO), Sall4 Zinc Finger Cl...
ORGANISM(S): Mus musculus 
2021-01-05 | E-MTAB-9197 | biostudies-arrayexpress
TranscriptomicsTimecourse RNA-seq of mouse embryonic stem cells during neural differentiation
Sall4 is a stem cell factor which is important for embryogenesis. We have genetically modified Sall4 in mouse embryonic stem cells to access the transcriptional changes. There are three different genetic modifications for the ES cells in the form of Sall4 Knockout (KO), Sall4 Zinc Finger Cluster 4 M...
ORGANISM(S): Mus musculus 
2021-01-05 | E-MTAB-9198 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of Sall4 mutants in mouse embryonic stem cells
Sall4 is a stem cell factor which is important for embryogenesis. We have genetically modified Sall4 in mouse embryonic stem cells to access the transcriptional changes. There are three different genetic modifications for the ES cells in the form of Sall4 Knockout (KO), Sall4 Zinc Finger Cluster 4 M...
ORGANISM(S): Mus musculus 
2021-01-05 | E-MTAB-7655 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of mouse embryonic stem cells in response to changes in Sall4
Sall4 is a stem cell factor which is important for embryogenesis. We have genetically modified Sall4 in mouse embryonic stem cells to access the transcriptional changes. There are three different genetic modifications for the ES cells in the form of Sall4 Knockout (KO), Sall4 Zinc Finger Cluster 4 M...
ORGANISM(S): Mus musculus 
2021-01-05 | E-MTAB-7343 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of mouse embryonic stem cells after knockout and re-introduction of Sall4a
Sall4 is a stem cell factor which is important for embryogenesis. In order to address transcriptional changes, we re-introduce Sall4 in homozygous Sall4 knockout (KO) mouse embryonic stem cells through random integration of Sall4 cDNA using a Piggybac vector which is Dox inducible. We also re-introd...
ORGANISM(S): Mus musculus 
2021-01-05 | E-MTAB-9202 | biostudies-arrayexpress
TranscriptomicsATAC-seq of mouse embryonic stem cells
To identify the accessible chromatin in wild-type mouse embryonic stem cells (mESCs), we performed ATAC-seq in mESCs.
ORGANISM(S): Mus musculus 
2021-01-05 | E-MTAB-9245 | biostudies-arrayexpress
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