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ProteomicsTMT-based quantitative proteomics to study CHN-1/HSP-1 complex substrate turnover in C. elegans
The wild type (WT) and HSP-1 EEYD (worms generating endogenously mutated HSP-1) C. elegans were cultured under normal conditions (20 degrees) or exposed to 30 degrees for 16 hours as L4 larvae (referred to as heat stress/HS). In WT worms undergoing HS, the activity of CHN-1 ubiquitin ligase is antic...
ORGANISM(S): Caenorhabditis elegans 
2024-11-08 | PXD048200 | Pride
E3 ligase/chaperone C. elegans Chn-1 (human ortholog chip) Hsp-1 (human ortholog hsp-70) 
TranscriptomicsSub1 occupancy in yeast cells in exponential growth condition
A genome-wide location analysis by ChIP on chip of the gene occupancy by Sub1 was undertaken to define the gene targets of Sub1 in vivo. Chromatin immunoprecipitation (ChIP) assays were performed on epitope-tagged Sub1-3HA cross-linked chromatin from exponentially growing cells. Immunopurified DNA a...
ORGANISM(S): Saccharomyces cerevisiae 
2009-08-27 | E-GEOD-11054 | biostudies-arrayexpress
TranscriptomicsGenome-wide analysis of gene expression and intron retention during development in a U12-type splicing-deficient zebrafish mutant [RNA-seq]
To determine the global impact of the clbn mutation on gene expression and efficiency of U2- and U12-type splicing, we analyzed the transcriptome of 108hpf wt and clbn mutant larvae by microarrays and RNA sequencing. RNAseq data was used to characterize intron retention of U2-type and U12-type intro...
ORGANISM(S): Danio rerio 
2014-02-05 | E-GEOD-53758 | biostudies-arrayexpress
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