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TranscriptomicsExpression profiling of E. coli rpoS knock-out strain during stationary phase in M9 minimal media with 0.2% glucose
Investigation of whole genome gene expression level in E. coli rpoS knock-out strain grown up to stationary phase in M9 minimal media supplemented with 0.2% glucose E. coli rpoS deletion mutant grown up to OD600nm 1.5 (stationary phase) in M9 minimal media supplemented with 0.2% glucose. The high-de...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2014-02-05 | E-GEOD-46543 | biostudies-arrayexpress
TranscriptomicsChIP-chip of E. coli K-12 MG1655 with antibody against RNA polymerase subunits, RpoB, RpoD, RpoS,RpoH, RpoN, FliA, and FecI
We investigate sigma factor binding regions for each sigma factor under various enviromental and/or genetic conditions. To measure sigma factor binding at a genome scale, we employed a ChIP-chip method to derivative strains of E. coli K-12 MG1655 wild type and its isogenic rpoS and rpoN knock-out s...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2014-02-05 | E-GEOD-46541 | biostudies-arrayexpress
TranscriptomicsGenome-scale reconstruction of the sigma factor network in E. coli
We report the application of single-molecule-based sequencing technology for high-throughput profiling of transcription start sites for Escherichia coli under different conditions. By obtaining sequence from 5' RACE (rapid amplification of cDNA ends) followed by deep sequencing, we generated genome-...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2014-02-05 | E-GEOD-46737 | biostudies-arrayexpress
GenomicsIn vivo probing of the DNA-binding Architecture by bacterial arginine repressor
Although DNA motifs recognized by the transcription factors (TFs) have been determined, challenges remain in probing in vivo architecture of TF-DNA complexes on a genome-wide scale. Here, we show in vivo architecture of Escherichia coli arginine repressor (ArgR)-DNA complexes using chromatin immunop...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2015-04-01 | E-GEOD-60546 | biostudies-arrayexpress
TranscriptomicsChIP-chip of E. coli K-12 MG1655 with antibody against PurR-8myc under various conditions.
We integrated transcription factor binding regions and mRNA transcript abundance to elucidate the PurR regulon experimentally. To measure transcription factor binding at a genome scale, we employed a ChIP-chip method to derivative strains of E. coli K-12 MG1655 harboring PurR-8myc under various con...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2011-06-14 | E-GEOD-26589 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis of Geobacter sulfurreducens under multiple growth conditions
Investigation of comprehensive information about the transcripts (boundary, level, etc.) across the entire G. sulfurreducens genome in mulitple growth conditions, including in biofilm and on electrode. A five array study using total RNA recovered from two separate culture conditions of G. sulfurred...
ORGANISM(S): Geobacter sulfurreducens PCA 
2010-07-01 | E-GEOD-22497 | biostudies-arrayexpress
TranscriptomicsChIP-chip of Geobacter sulfurreducens PCA with antibody against RNAP and RpoD under various conditions
We applied a ChIP-chip approach to elucidate the binding profiles of RNAP and RpoD experimentally under different growth conditions. This technique localizes DNA fragments within DNA-protein complexes enriched by chromatin immunoprecipitation using high-density oligonucleotide tilling arrays. A 21 C...
ORGANISM(S): Geobacter sulfurreducens PCA 
2010-07-01 | E-GEOD-22503 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis of E. coli MG1655
Expression profiling of wild type and purR deletion strains of E. coli K-12 MG1655 under both M9 minimal media and addition of adenine. An eight chip study with two different strains under two separate culture conditions.
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2011-06-14 | E-GEOD-26588 | biostudies-arrayexpress
TranscriptomicsGenome-wide transcription start site determination of Geobacter sulfurreducens under multiple growth conditions
Determine the transcription start sites at 1-bp resolution across the entire G. sulfurreducens genome in multiple growth conditions, including fresh water acetate-fumarate (FWAF) medium, nitrogen limitation and growth on electrode. Special capture of the 5' end of primary transcripts in G. sulfurred...
ORGANISM(S): Geobacter sulfurreducens PCA 
2010-07-01 | E-GEOD-22511 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis of E.coli MG1655 under multiple growth conditions
Investigation of comprehensive information about the expression level of RNA transcripts across the entire E.coli genome in mulitple growth conditions, including log-phase; stationary phase, heat shock and nitrogen-limiting condition. A fourteen chip study using total RNA recovered from four separa...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2009-11-03 | E-GEOD-15534 | biostudies-arrayexpress
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