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GenomicsGlobal analysis of in vivo EGR1-binding sites in erythroleukemia cell K562 using chromatin immunoprecipitation and massively parallel sequencing
Early growth response gene 1 (EGR1) has been implicated in megakaryocyte differentiation induced by PMA (phorbol 12-myristate 13-acetate). The identification of direct EGR1 target genes in global scale is critical for our understanding of how EGR1 contributes to this process. In this study, we provi...
ORGANISM(S): Homo sapiens 
2010-09-08 | E-GEOD-21665 | biostudies-arrayexpress
TranscriptomicsChromatin Immunoprecipitation of UV treated AdHBx cells with anti-HBx antibodies
Direct gene targets of HepG2 cells transduced with Adenoviral vector carrying HBx vs Adenoviral vector under UV treatment Keywords: ChIP-chip profiling One-condition experiment, AdHBx (experimental) UV vs AdEasy (vector control) UV. HepG2 cells were infected with either recombinant HBx adenoviruses...
ORGANISM(S): Homo sapiens 
2008-07-01 | E-GEOD-11106 | biostudies-arrayexpress
GenomicsAHT-ChIP-seq, a completely automated robotic protocol to perform chromatin immunoprecipitation in high-throughput
Transcription factor binding locations by ChIP followed by high throughput sequencing. To build and validate an automated Chromatin Immunoprecipitation and high throughput Illumina sequencing pipeline
ORGANISM(S): Homo sapiens 
2013-06-01 | E-MTAB-1579 | biostudies-arrayexpress
GenomicsGenome-wide profiles of STAT1 DNA association using chromatin immunoprecipitation and massively parallel sequencing
We developed a method, ChIP-sequencing (ChIP-seq), combining chromatin immunoprecipitation (ChIP) and massively parallel sequencing to identify mammalian DNA sequences bound by transcription factors in vivo. We used ChIP-seq to map STAT1 targets in interferon stimulated and unstimulated human HeLa S...
ORGANISM(S): Homo sapiens 
2009-03-23 | E-GEOD-15353 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of human HNF3b and E2F4, and mouse NeuroD1, for THEME analysis
Genome-wide chromatin-immunoprecipitation (ChIP-chip) detects binding of transcriptional regulators to DNA in vivo at low resolution. Motif discovery algorithms can be used to discover sequence patterns in the bound regions that may be recognized by the immunoprecipitated protein. However, the disco...
ORGANISM(S): Mus musculus 
2006-05-09 | E-WMIT-8 | biostudies-arrayexpress
TranscriptomicsA Chromatin Immunoprecipitation Approach Based on BAC Transgenesis
We have devised a technology that enables chromatin immunoprecipitation (ChIP) experiments in cultured human cells by circumventing both the laborious production of protein-specific antibodies and the optimization of crosslinking/immunoprecipitation conditions for each protein. Our approach utilizes...
ORGANISM(S): Homo sapiens 
2010-05-25 | E-GEOD-10845 | biostudies-arrayexpress
TranscriptomicsGenomic distribution of CHD7 on chromatin tracks H3K4 methylation patterns
CHD7 is a member of the chromodomain helicase DNA binding domain family of ATP-dependent chromatin remodeling enzymes. De novo mutation of the CHD7 gene is a major cause of CHARGE syndrome, a genetic disease characterized by a complex constellation of birth defects. To gain insight to the function...
ORGANISM(S): Mus musculus 
2009-01-22 | E-GEOD-14460 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of centromeric proteins CenpA and CenpC from human cell lines containing a neocentromere in band 13q21
Chromatin Immunoprecipitation of Centromeric Proteins, CenpA or CenpC, from human cell lines containing a neocentromere in band 13q21
ORGANISM(S): Homo sapiens 
2006-11-06 | E-TABM-168 | biostudies-arrayexpress
ProteomicsPfMORC protein regulates chromatin accessibility and transcriptional repression in the human malaria parasite, P. falciparum.
Enrichment of PfMORC-HA associated proteins by HA immunoprecipitation from P. falciparum parasites expressing P. falciparum MORC tagged with a 3xHA tag.
ORGANISM(S): Plasmodium Falciparum (ncbitaxon:5833) 
2023-07-06 | MSV000092353 | MassIVE
TranscriptomicsAnalysis of HSF2 target genes using a global promoter ChIP-chip strategy on mouse wild-type testis
Mapping of in vivo targets for HSF2 by using mammalian tissue material in a global ChIP-chip promoter screen. Chromatin was isolated from three wild-type (WT) mouse testes, crosslinked and sonicated into fragments of 100-500 bp, and the quality of the DNA was controlled before the immunoprecipitatio...
ORGANISM(S): Mus musculus 
2008-08-05 | E-GEOD-9289 | biostudies-arrayexpress
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