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OtherHuman spermatozoa as a model for detecting missing proteins in the context of the chromosome-centric Human Proteome Project.
Data from ProteomeXchange, PXD ID: PXD002367. File: Mitchell_40_F03_exclu02.mzml. Published as part of J Proteome Res. 2015 . From the Abstract: {{i}} The chromosome-centric Human Proteome Project (C-HPP) aims at cataloguing the proteins as gene products encoded by the human genome, in a chromosome...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM32320016359 | GPMDB
Resource Reanalysis 
ProteomicsUpdate on virtual-experimental 2DE approach in chromosome-centric human proteome project
In the boundaries of chromosome-centric Human Proteome Project c-HPP to obtain information about proteoforms coded by chromosome 18, several cell lines were analyzed. In our study we have been using proteoform separation by 2DE (sectional analysis) and semi-virtual 2DE with following shotgun mass-sp...
ORGANISM(S): Homo sapiens (Human) 
2018-09-24 | PXD010142 | Pride
Esi lc-ms/ms Inventory Proteoforms Mass spectrometry Chromosome 18 Chromosome-centric Two-dimensional electrophoresis Proteome 
ProteomicsProteomic and phosphoproteomic data of colorectal cancer tissues and cells for Chromosome-Centric Human Proteome Project
Protein extraction and proteolytic digestion were performed using a Filter-Assisted Sample Preparation (FASP) protocol. In case of phosphopeptide enrichment immobilized Fe(III) affinity chromatography (Fe-IMAC) was performed. The peptides were fractionated using an HPLC system fitted with an SCX col...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-15 | MSV000079583 | MassIVE
ProteomicsUpdate on virtual-experimental 2DE approach in chromosome-centric human proteome project
In the boundaries of chromosome-centric Human Proteome Project c-HPP to obtain information about proteoforms coded by chromosome 18, several cell lines were analyzed. In our study we have been using proteoform separation by 2DE (sectional analysis) and semi-virtual 2DE with following shotgun mass-sp...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-11-12 | MSV000086448 | MassIVE
OtherIdentification of missing proteins defined by chromosome-centric proteome project in the cytoplasmic detergent-insoluble proteins
Data from ProteomeXchange, PXD ID: PXD001694. File: lungSILAC_first_14.mzml. Published as part of J Proteome Res. 2015 Jun 25 . From the Abstract: {{i}} ... As most samples prepared and analyzed in the C-HPP framework were focusing on detergent soluble proteins, we posit that as a natural compositi...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM32320016148 | GPMDB
Resource Reanalysis 
ProteomicsChromosome 11-Centric Human Proteome Project
For the analysis of chromosome 11-centric human proteome, we analyzed the human brain hippocampal proteome. The samples from brain tissues were extensively off-gel fractionated by 12 fractions and analyzed by a high resolution mass spectrometer, Orbitrap XL ETD with collision induced dissociation...
ORGANISM(S): Homo sapiens (Human) 
2016-10-10 | PXD000395 | Pride
Offgel fractionation Chromosome 11 Pe fraction Human brain hippocampus tissues 
ProteomicsProteogenomics reanalysis of human testis data
Reanalysis of the human testis tissue dataset acquired for the Chromosome-centric Human Proteome Project (C-HPP; PRIDE project PXD002179), with a focus on identifying novel peptides for genome annotation.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-06-13 | MSV000083973 | MassIVE
ProteomicsEnrichment strategy for searching missing protein
Enrichment strategy for searching missing protein is a project under the guidance of Chromosome-Centric Human Proteome Project (C-HPP). We developed systematical enrichment strategies for revealing the existence of MPs including low molecular weight (LMW), membrane proteins, post-translational modif...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-06-12 | MSV000083957 | MassIVE
ProteomicsThe human spermatozoa proteome
The human spermatozoa proteome was in depth characterized using shotgun iterative GeLC-MS/MS method with peptide exclusion lists.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-06-26 | MSV000082508 | MassIVE
ProteomicsMissing proteins identified from the haploid cell lines: HAP1 and KBM-7
Chromosome-centric Human Proteomic Project (C-HPP) aims at identifying and characterizing protein products encoded from all protein-coding genes of human chromosomes. As evident in the recent nature papers, it is possible that those missing proteins may be low abundant in many cell types or expresse...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-06-10 | MSV000083952 | MassIVE
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